脱氧核糖核酸酶I中英文说明书

Enzymatic Reaction： 

Bovine pancreatic deoxyribonuclease is an endonuclease that preferentially splits phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5’-phosphate terminated polynucleotides with a free hydroxyl group at the 3’ position. DNase I is secreted by exocrine glands, and found most abundantly in the pancreas and parotid. It is also present in lower quantities in other tissues (Chen and Liao 2006, and Nadano et al. 1993).

DNase is known to be involved in apoptosis and has been proposed to play a role in the regulation of actin polymerization in cells. In addition to its use in molecular biology, DNase I has been used as a treatment for cystic fibrosis, and systemic lupus erythematosus (Chen and Liao 2006).

Specificity:

bpDNase I is not base nor sequence specific; however, it does not cleave randomly. It shows preference for cleavage at the 5’ side of pyrimidines, and is particularly pronounced in alternative copolymers (Bernardi et al. 1975, and Lomonossoff et al. 1981). It has been shown that variations in the twist angle are recognized by DNase I (Dickerson and Drew 1981). The specificity of DNase I also depends on the divalent cations present. In the presence of Ca2+ and Mg2+, it causes single strand breaks, and in the presence of Mn2+ double strand breaks have been reported (Junowicz and Spencer 1973, and Campbell and Jackson 1980).

Applications:

DNA removal in primary cell isolation: decreases viscosity providing better yields

DNA removal in bioprocessing applications

Removing genomic DNA from RNA preparations prior to RT-PCR

in vitro transcription

Nick translation

DNase footprinting

Actin binding

UV crosslinking of proteins to nucleic acids

Radioactive labeling

艾美捷Worthington脱氧核糖核酸酶I特异性：

bpDNase I 不是碱基或序列特异性的；但是，它不会随机切割。它显示优先在嘧啶的 5' 侧进行裂解，并且在替代共聚物中尤其明显（Bernardi 等人 1975 和 Lomonossoff 等人 1981）。已经表明，扭曲角的变化被 DNase I 识别（Dickerson 和 Drew 1981）。DNase I 的特异性还取决于存在的二价阳离子。在存在 Ca2+ 和 Mg2+ 的情况下，它会导致单链断裂，而在存在 Mn2+ 的情况下会导致双链断裂（Junowicz 和 Spencer 1973，以及 Campbell 和 Jackson 1980）。

艾美捷Worthington脱氧核糖核酸酶I分子特征：

与人类、小鼠和大鼠类似，牛胰腺 DNase I 基因由 9 个外显子组成，只有最后 8 个外显子编码该蛋白质（De María 和 Arruti 2003）。新生蛋白通过由外显子二编码的 22 个氨基酸信号序列引导至分泌途径细胞器。主要活性位点残基 H166 由外显子 6 编码（De María 和 Arruti 2003 和 Kraehenbuhl 等人 1977）。尽管牛和其他哺乳动物的外显子长度几乎相同，但内含子的长度差异很大。TATA 盒序列位于外显子 I 上游 35 bp（De María 和 Arruti 2003）。已经提出影响编码序列的多重剪接事件可能是下调 DNase I 表达的机制（Liu 等人 1997）。

脱氧核糖核酸酶I相关研究：

肌动蛋白

白蛋白，无核酸酶

脱氧核糖核酸酶 II

脱氧核糖核酸及相关产品

组蛋白

溶菌酶

核酸酶，微球菌

核酸酶，S1

磷酸酶，碱性

磷酸二酯酶 I

磷酸二酯酶 II

蛋白酶K

逆转录酶，重组 HIV

核糖核酸酶A

核糖核酸酶 T1

核糖核酸酶，E-Rase RNase 混合物

核糖核酸