脱氧核糖核酸酶I中英文说明书

Bovine pancreatic deoxyribonuclease I produced recombinantly in yeast, Pichia pastoris, to decrease levels of contaminating RNase and eliminate potential pathogens associated with animal based materials.

Bovine pancreas is a rich source of RNase A which is often found in many commercial DNase preparations. Producing DNase I by recombinant means in an organism with much lower levels of endogenous RNase greatly facilitates purification of an enzyme with undetectable levels of RNase. The processes involved in the production and isolation of recombinant DNase I are completely devoid of animal based components which eliminates the possibility of introducing animal derived pathogens into bioprocessing procedures.

Recombinant DNase I is suitable for such applications as:

• Removing genomic DNA from RNA preparations prior to RT-PCR

• Degradation of DNA templates after transcription reactions

• Removing unwanted DNA from samples prior to Northern blotting

• Removing DNA during biopharma and bioprocessing procedures

Unit Definition: One Unit causes an increase in absorbance at 260nm of 0.001 per minute at 25oC when acting upon highly polymerized DNA at pH 5.0, which is the same as other Worthington DNase I products.

Note: Kunitz units as reported by other suppliers can be 2 to 4 times higher than Kunitz units as measured at Worthington. As measured at Worthington, One Kunitz unit digests 1mg of calf thymus (or pUC19 or l-phage) DNA in 10 minutes at 37oC in 50mM Tris, 1mM Mg2+, 1mM Ca2+, pH 7.8. Correlation of digestion units with Kunitz units may be different in other buffer systems.

Storage Buffer (DR1S): 5mM calcium acetate, 4mg/ml glycine, pH 5.0 and 50% glycerol.

DNase I Reaction Buffer (10X): 500mM Tris-HCl, 10mM MgSO4, 1mM CaCl2, pH 7.8, provided.

艾美捷Worthington 脱氧核糖核酸酶I特异性：

bpDNase I 不是碱基或序列特异性的；但是，它不会随机切割。它显示优先在嘧啶的 5' 侧进行裂解，并且在替代共聚物中尤其明显（Bernardi 等人 1975 和 Lomonossoff 等人 1981）。已经表明，扭曲角的变化被 DNase I 识别（Dickerson 和 Drew 1981）。DNase I 的特异性还取决于存在的二价阳离子。在存在 Ca2+ 和 Mg2+ 的情况下，它会导致单链断裂，而在存在 Mn2+ 的情况下会导致双链断裂（Junowicz 和 Spencer 1973，以及 Campbell 和 Jackson 1980）。

脱氧核糖核酸酶I相关研究：

肌动蛋白

白蛋白，无核酸酶

脱氧核糖核酸酶 II

脱氧核糖核酸及相关产品

组蛋白

溶菌酶

核酸酶，微球菌

核酸酶，S1

磷酸酶，碱性

磷酸二酯酶 I

磷酸二酯酶 II

蛋白酶K

逆转录酶，重组 HIV

核糖核酸酶A

核糖核酸酶 T1

核糖核酸酶，E-Rase RNase 混合物

核糖核酸