Study on modified triphosphate: lumiprobe amino-11-ddutp

AI Meijie Lumiprobe Amino-11-ddUTP  It is a terminator triphosphate , be used for  DNA  Enzyme end labeling . Nucleotides pass through  Taq DNA  Polymerase 、Sequenase、Klenow  Fragment incorporation . Amino terminal  DNA  The subsequent reaction with the activated ester can be used for  DNA  End mark of .

AI Meijie  Lumiprobe amino -11-ddUTP chemical property ：

form ：  Colorless solid

Molecular weight ：960.44

CAS Number ： 439077-17-5（ Free of cations and crystal water ）

Molecular formula ：C 18 H 62 Li 3 N 4 O 32 P 3

solubility ： Good water

The quality control ：NMR 1 H  and 31 P, HPLC-MS (99+%)

Storage conditions ： Store ：-20°C  24  Months . transport ： At room temperature for  3  Zhou . dry .

AI Meijie Lumiprobe  amino -11-ddUTP Related literature ：

1、Bakker, R.; Mani, M.; Carthew, R.W. The Wg and Dpp morphogens regulate gene expression by modulating the frequency of transcriptional bursts. bioRxiv, preprint. doi: 10.1101/2020.01.24.918623

2、Boersma, S.; Rabouw, H.H.; Bruurs, L.J.M.; Pavlovič, T.; van Vliet, A.L.W.; Beumer, J.; Clevers, H.; van Kuppeveld, F.J.M.; Tanenbaum, M.E. Translation and Replication Dynamics of Single RNA Viruses. Cell, 2020, 183(7), 1930–1945.e23. doi: 10.1016/j.cell.2020.10.019

3、Yu, Y.; Guo, Y.; Tian, Q.; Lan, Y.; Yeh, H.; Zhang, M.; Tasan, I.; Jain, S.; Zhao, H. An efficient gene knock-in strategy using 5'-modified double-stranded DNA donors with short homology arms. Nature Chemical Biology, 2020, 16(4), 387–390. doi: 10.1038/s41589-019-0432-1

Lumiprobe amino -11-ddUTP Related research ：

amino -11-dUTP

Amino-11-dUTP  It is used to decorate  DNA  Triphosphate .

amino -11-UTP

Uridine triphosphate labeled with amino group , It is used for indirect non radioactive enzyme catalysis  RNA  Mark .

Biotin -11-dCTP

For non radioactive  DNA  Labeled biotinylation  dCTP.

Biotin -11-dUTP

Biotin labeled deoxyuridine triphosphate , For enzymatic incorporation  DNA  And indirect detection .

Biotin -11-UTP

For enzymatic  RNA  Labeled biotinylation  UTP.

FAM-11-dCTP,6- Isomer

For enzymatic  DNA  Of the tag  6-FAM  Of the tag  dCTP.

FAM-11-dUTP,6  Isomer

be used for  DNA  Marked with green emission  dUTP  Of  FAM  derivatives .

FAM-11-UTP,6  Isomer

6-FAM- Uridine triphosphate is used as a non radioactive enzyme in the process of transcription in vitro  RNA  Mark .