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Pal2Nal|如何在命令行下运行Pal2Nal
2022-06-21 20:18:00 【name_qgy】
1.Pal2Nal是什么
Pal2Nal是一个将已经比对好的蛋白及其对应的DNA(mRNA)多序列转化为密码子比对的程序。有在线网页版本,有Linux操作系统下的命令行版本,本文介绍的是命令行版本。
Pal2Nal的官方网址:
http://www.bork.embl.de/pal2nal/
2.Pal2Pal的下载安装
conda create -n pal2nal
conda activate pal2nal
conda install pal2nal
- 运行Pal2Nal
perl pal2nal.pl pep.fas nuc.fas -output codon > ./*.fas -codontable number
#输入文件,两个,分别是比对好的PEP和原始的CDS序列
#-output 输出文件格式,默认为clustal
#-output + ' ' + > + ' ' +输入的路径及文件名
#-codontable 选择合适的密码子表
#pal2nal -h 可以看到详细的参数信息
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