Application and chemical properties of elastase

Elastase (EC3.4.4.7,Elastase) It is an endopeptidase containing serine , Its effect on glycine 、 Alanine residues have high hydrolysis specificity . Because glycine in elastin 、 The content of alanine reaches 55% above , Therefore, elastase can efficiently hydrolyze alanine 、 Leucine et al J Insoluble elastin with reticular structure, which is crosslinked by amino acid residues (Elastin).

Elastase has anti arteriosclerosis properties 、 Effect of improving lipid metabolism , It can be used to prevent atherosclerosis , Treat hyperlipidemia 、 Biochemical drugs for fatty liver [2], Effective 、 Safe and reliable .

meanwhile , Its high-efficiency hydrolysis of elastin can also be used as a tenderizing agent in the food industry , Used as detergent in daily chemical industry , It has broad application prospects and high commercial value .

Distribution and source of elastase elastase is mainly distributed in animals and some microorganisms , Some plants may also have elastase active substances .

Use of elastase ：

It is mainly used to treat hyperlipidemia in medical clinic , Prevent atherosclerosis 、 Fatty liver disease . It can also be used to tenderize meat and aquatic products .  

AI Meijie Worthington Application of elastase ：

Tissue dissociation ： Because elastin has the highest concentration in the elastic fibers of connective tissue , Therefore, elastase is often used to dissociate tissues containing extensive intercellular fibrous networks . So , It is usually used with other enzymes , For example, collagenase 、 Trypsin and chymotrypsin .

AI Meijie Worthington Chemical properties of elastase ：

Chinese name ： Elastase , Purified

An English name ：Elastase, Purified

cat#：WBC-LS006367

specifications ：Bulk

stability / Store ： Elastase is in  pH ≤ 3.5  Unstable when . When stored as dry powder , Enzyme in  2-8°C  It can be stored stably under  6-12  Months . Elastase product code ：ES  and  ESL  In neutral  pH  And concentrations greater than  0.25%  Poor solubility at . Before diluting into the reaction mixture or medium , stay  KCl  It is helpful to prepare the primary solution in alkaline buffer , To compensate for ionic strength or  pH  Change in value . stay  pH 4.0-10.4  Stable .

Technical specification ： 1  individual  SucAla 3 NA  The unit is approximately equal to  6  An elastin digestion unit . Aqueous liquid suspensions should be treated aseptically to avoid bacterial contamination . Due to water suspension （ Code ：ES） Viscosity of , The vial should be rinsed to recover its contents .

Unit definition ： A unit in  25°C、pH 8.0  Crack every minute  1  Micromore  N- Succinyl -L- Alanyl -L- Alanyl -L- Alanine - P-nitroaniline .