Characteristics and related specificity of Papain

Papain is an active enzyme in papaya , It is for the human body , It can improve immunity , Antiphlogistic and analgesic , Promoting digestion , in addition , It can relieve itching and pain caused by mosquito bites , And can promote the healing of trauma , Papain can also be used to prevent scars, which has achieved a better therapeutic effect .

So papain can be used properly , Play the role of nutrition and health care , But it is not the same as medicine , So we can't blindly believe its therapeutic effect , Don't use... Blindly .

AI Meijie Worthington Characteristics of Papain ：

Papain is a kind of protease in acid 、 Neutral 、 Protease that can decompose protein in alkaline environment . Its appearance is white to light yellow powder , Slightly hygroscopic ; Papain is soluble in water and glycerin , The aqueous solution is colorless or light yellow , Sometimes milky white ; Almost insoluble in B chun、lv Imitation and B mi And other organic solvents . Papain is a sulfhydryl group (-SH) Endopeptidase , It has the activity of protease and esterase , It has a wide range of specificity , For animal and plant proteins 、 Polypeptides 、 ester 、 Amides have strong hydrolysis ability , But it can hardly break down peptone .

Papain zui fit PH value 6～7( commonly 3～9.5 All can ), It also works when it is neutral or acidic , Isoelectric point （pI） by 8.75; The most suitable temperature for papain 55～65℃( commonly 10～85℃ All can ), Strong heat resistance , stay 90℃ It will not be completely inactivated ; Inhibited by oxidants , Reducing substance activation .

features ：

Papain is mainly used for cold resistance of beer （ Hydrolyze the protein in beer , Avoid turbidity caused by cold storage ）、 Meat softening （ Hydrolyzed muscle protein and collagen , Soften meat ）、 Preparation of pre cooked cereals 、 Production of hydrolyzed protein and meat flavor . It is widely used in beer cold resistance and meat softening than other proteases .

AI Meijie Worthington Papain specificity ：

Papain has a wide range of specificity ; It has endopeptidase 、 Amidase and esterase activity . The active site consists of seven sub sites （S 1 -S 4 and  S 1' -S 3'） form , Each subunit can hold one amino acid residue of the substrate （P 1 -P 4 and  P 1' -P 3'）（Schechter And Berger  1967）. Specificity is determined by  S 2 Sub site control ,S 2  A subsite is a host substrate  P 2 Side chain hydrophobic bag . Papain exhibits a specific substrate preference , It is mainly due to a large number of hydrophobic or aromatic residues at this sub site （Kimmel  and  Smith 1954）. stay  S 2 Extra loci preference , Lack of well-defined residue selectivity within the active site .

AI Meijie Worthington Papain chemical property ：

Chinese name ： Papain , Suspension

An English name ：Papain, Suspension

cat#：WBC-LS003128

specifications ：Bulk

stability / Store ： stay  2-8°C  It can be stored stably under  6-12  Months . Do not freeze aqueous suspensions .

Technical specification ： Papain preparation should be incubated in activation solution before use , To ensure full activation . Applications include antibody fragmentation and primary generation / Separation of nerve cells .

Unit definition ： It contains  1.1 mM EDTA、0.067 mM  Mercaptoethanol and  5.5 mM  Cysteine -HCl  Activated in a solution of  30  Minutes later , A unit in  25°C、pH 6.2  Hydrolysis per minute under conditions  1  Micromolar benzoyl -L- Arginine ethyl ester .