Amino dye research: lumiprobe fam amine, 6-isomer

Fluorescein derivatives with amino groups , Contains pure 6- Fluorophores of isomers . The reagent can be used to modify biomolecules by enzymatic amino transfer . Its aliphatic amine groups are also linked to electrophilic reagents （ Such as activated ester ） React . This amine can also be reduced by amination with carbonyl compounds （ Aldehydes and ketones ） conjugate .

AI Meijie Lumiprobe FAM  amine ,6- Chemical properties of isomers ：

form ：  Yellow solid

Molecular weight ：510.97

CAS Number ： 2183440-42-6（ Hydrochloride ）、1313393-44-0

Molecular formula ：C 27 H 27 N 2 ClO 6

IUPAC  name ：5- Aminohexylaminocarbonyl fluorescein hydrochloride

solubility ： Good methanol 、DMSO、DMF

The quality control ：NMR 1 H, HPLC-MS (95%)

Storage conditions ： Store ： stay  -20°C  Keep away from light . transport ： At room temperature for  3  Zhou . Avoid prolonged exposure to light . dry .

AI Meijie Lumiprobe FAM  amine ,6- Spectral properties of isomers ：

The greatest inspiration / absorption ,nm：490

ε, L⋅mol -1 ⋅cm -1：80000

The largest launch , nanometer ：513

Fluorescence quantum yield ：0.93

CF 260： 0.20

CF 280： 0.17

AI Meijie Lumiprobe  Sulfo cyanine 7 Dicarboxylic acid Related research ：

Sulfo cyanine 7 amine

Water soluble  NIR  Dye amine ,Cyanine7  Sulfonated derivatives of dyes , For modification by enzymatic amino transfer .

Sulfo cyanine 5 amine

Sulfonated  Cyanine5  Amine derivatives , It is used for coupling with electrophilic reagent and enzymatic transamination labeling .

Sulfo-Cyanine5.5  amine

Sulfo-Cyanine5.5  It is a kind of far red / Near infrared fluorophore , It has high hydrophilicity and water solubility . Due to the excellent molar extinction coefficient , The dye shows good brightness in the far red area . This is an amine derivative that is reactive to electrophilics . It can also be used for enzymatic transamination labeling .

Sulfo cyanine 3 amine

Water soluble cyanine dye , Bright fluorescence , High extinction coefficient . Contains amino and enzymatic amino transfer markers for binding to electrophilic agents .

FAM  amine ,5- Isomer

Amino derivatives of fluorescein , pure  5- Isomer . There is a connecting arm between the aliphatic amine group and the fluorophore . It can be used for labeling by enzymatic amino transfer 、 Reaction with electrophilic reagents and reductive amination .

TAMRA  amine ,5- Isomer

TAMRA（ Tetramethylrhodamine ） dyestuff , pure  5- Isomer , With an amino group , It can be used with electrophilic reagents （NHS  ester 、 Epoxide, etc ） combination .

BDP TR amine

Having free amino groups  BDP TR  Boron Dipyrrole methylene dye , It is used for coupling with electrophilic reagent and enzymatic transamination reaction .BDP TR  yes  ROX  The bright dye of the channel .

BDP TMR  amine

BDP TMR amine  It's a  TAMRA  The amino dye of the channel . The dye has bright fluorescence , It is very suitable for fluorescence polarization measurement .