Testing principle and precautions of biovendor rage ELISA Kit

AI Meijie  BioVendor RD191116200R sRAGE Elisa A kit is a kind of kit for people sRAGE（ Late glycation terminal soluble receptor ） Quantitative determination tool .

AI Meijie  BioVendor sRAGE Elisa Kit Test principle ：

stay Biovendor Human sRAGE ELISA in , Standards 、 Quality control and samples have been incubated in microplates precoated with polyclonal anti human sRAGE antibody .120 Incubate and wash after minutes , Add biotin labeled polyclonal anti human sRAGE Antibodies and captured sRAGE Incubate 60 minute . After cleaning again , Streptavidin HRP Add conjugate . stay 30 Minutes after incubation and the last washing step , The remaining allowed conjugate and substrate solution （TMB） reaction . The reaction was measured for the absorbance of the added acid solution and the resulting yellow product . This absorbance is similar to sRAGE Is proportional to the concentration of . The standard curve consists of plotting the absorbance value and the standard sRAGE Concentration and use this standard curve to determine unknown samples .

AI Meijie  BioVendor sRAGE Elisa Kit matters needing attention ：

− For professional use only

− When handling immunodiagnostic materials , Gloves and lab coats should be worn

− Do not drink alcohol in the area where immunodiagnostic materials are handled 、 Eating or smoking

− The kit contains components from human sources . It is found that these materials are right for HBsAg、HCV Antibodies and targets HIV Of 1/2 Antigens and antibodies . however , These materials should be considered potentially infectious , Because no test can guarantee that there is no source of infection

− This kit contains ingredients of animal origin . These materials should be treated for potential infectivity in the following manner

− Avoid contact with hydrogen containing acidic stopping solutions and base solutions peroxides and tetramethylbenzidine （TMB）. Wear gloves when 、 Eye and clothing protection handle these reagents . Stop and / Or matrix solution may cause skin / Eye irritation . In case of contact with the stop solution and the matrix solution , Please wash your skin thoroughly / Eyes if necessary , Rinse with water and seek medical attention

− Do not suck material through an oral pipette

AI Meijie  BioVendor sRAGE Elisa Kit related literature ：

1、Brett J, Schmidt AM, Yan SD, Zou YS, Weidman E, Pinsky D, Nowygrod R, Neeper M, Przysiecki C, Shaw A, et al. Survey of the distribution of a newly characterized receptor for advanced glycation end products in tissues. Am J Pathol. 1993 Dec;143 (6):1699-712

2、Emanuele E, D'Angelo A, Tomaino C, Binetti G, Ghidoni R, Politi P, Bernardi L, Maletta R, Bruni AC, Geroldi D. Circulating levels of soluble receptor for advanced glycation end products in Alzheimer disease and vascular dementia. Arch Neurol. 2005 Nov;62 (11):1734-6

3、Falcone C, Emanuele E, D'Angelo A, Buzzi MP, Belvito C, Cuccia M, Geroldi D. Plasma levels of soluble receptor for advanced glycation end products and coronary artery disease in nondiabetic men. Arterioscler Thromb Vasc Biol. 2005 May;25 (5):1032-7

AI Meijie  BioVendor sRAGE Elisa Kit related research ：

1、 protein

RD172116100-HEK

Soluble Receptor for Advanced Glycation End Products (sRAGE) Human HEK293（HEK293 Expressive person ）

RD172116100

Soluble Receptor for Advanced Glycation End Products Human E. coli（ E. coli expressing human ）

RD272590100

Soluble Receptor for Advanced Glycation End Products (sRAGE) Mouse HEK293（HEK293 Expressing mouse ）

2、 antibody

RD184116100

Soluble Receptor for Advanced Glycation End Products (sRAGE) Human, Sheep Polyclonal