Solve blast database error: error pre fetching sequence data

1、 background ：

download Uniprot From the protein sequence in the database to the local , Use this file to create BLAST Search database for , And then use blastp Command to search the database .

2、 error

according to blast Instructions in the manual , To speed up the search process , Use mask file . from Uniprot Downloaded sequence file sequences.fasta, The content is as follows ：

>sid1

xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

>sid2

xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

.................................................................

1） establish mask file ：

segmasker -in sequences.fasta -infmt fasta -outfmt maskingfo_asnl_bin \

-out refsequences.asnb -parse_seqids

2) Create database

makeblastdb -in sequences.fasta -input_type fasta -dbtype prot -out uniprot \

-title "Uniprot" -parse_seqids

3) Sequence alignment

blastp -db uniprot -query input.fasta -out output.txt -outfmt 7

The result is wrong ：BLAST Database error: Error pre-fetching sequences data

3、 resolvent

Creating mask Files and databases are not used parse_seqids.

segmasker -in sequences.fasta -infmt fasta -outfmt maskingfo_asnl_bin \

-out refsequences.asnb

makeblastdb -in sequences.fasta -input_type fasta -dbtype prot -out uniprot -title "Uniprot"

Why? ？ I don't know why , Looking up many articles on the Internet can't solve , I guess there is something wrong with creating the database , Try to remove unnecessary parameters one by one , It was found that parse_seqids After the parameter , Can use balstp Get the comparison results correctly .