Characteristics of exonuclease in Worthington venom and related literature

Venom exonuclease （ Phosphodiesterase  I） Continuous hydrolysis comes from  3'- Hydroxyl terminal ribose and deoxyribonucleic acid  5'- Single nucleotide .Laskowski, Sr. (1971)  It was reviewed .

The enzyme has been widely used as a tool for the study of nucleic acid structure and sequence （Ho  and  Gilham 1973）.

Worthington Crotalus adamanteus Characteristics of exonuclease in venom ：

 

Molecular weight ：115,000 (Philipps 1975).

composition ： A binding concanavalin  A  Glycoprotein .（Sulkowski  and  Laskowski 1961）.

The best  pH  value ：9.8-10.4 (Philipps 1975).

Inhibitors ： Reducing agent , Such as glutathione 、 Cysteine and ascorbic acid （Razell 1963）. It has been  5 mM EDTA  Completely inhibit , and  ATP、ADP  and  AMP  It is a partial inhibitor .

Activator ： Enzyme pair Mg 2+ Demand ;Philipps (1975)  It is pointed out that the optimal concentration is  15 mM.

Specificity ： The enzyme has been  Dolapchiev And others purify .(1974)  and  Sulkowski  and  Laskowski, Sr. (1961), Endonuclease activity and  5'- Nucleotidase and nonspecific monophosphatase were eliminated .

It is nonspecific to the base or sugar part of nucleotides . Various synthetic substrates are hydrolyzed . See  Razell (1963)  The comments .Ogilvie  and  Hruska (1976)  It is pointed out that exonuclease cannot recognize  syn  Nucleoside units in the conformation . See also  Philipps  and  Chiemprasert (1975).

ADP- Ribosylated proteins are cleaved by venom phosphodiesterase at the pyrophosphate bond , Produce phosphoribosyl -AMP (Hayaishi 1976).

AI Meijie Worthington Crotalus adamanteus Exonuclease in venom Related research ：

Albumin , Nuclease free  (BSANF)

Deoxyribonuclease  I (DP/D/DCLS/D2/DPFF/DPRF)

E•RASE RNase A/T1  Mixture  (RCT)

Histone  (H, NHL)

Lysozyme  (LY/LYSF)

Nuclease , Micrococcus  ( NFCP)

Nuclease ,S1 (SINUC/SINUCL)

nucleic acid ,DNA, E. coli ,λ/ fragment ,RNA

Phosphatase , alkalinity  (CAP/BAPF/BAPC/BAPSF/PC)

Phosphodiesterase  II (SPH)

protease  K (PROK/PROKS)

Reverse transcriptase , restructuring  HIV (RTHIV)

Ribonuclease  A (R/RAF/RASE/RS/RPDF)

Ribonuclease  T1, No animal origin  (RT1S)

Related literature ：

1.Bruns, M.  and  Philipps, G.： The effect of venom phosphodiesterase on E. coli aminoacyl -tRNA  The role of , Biochim Biophys Acta 217 , 189, 1970

2.Burgers, P.、Eckstein, F.  and  Hunneman, D.： Stereochemistry of phosphodiesterase hydrolysis of snake venom , J Biol Chem 254 , 7476, 1979

3.Cheung, W.: Cyclic 3',5'-Nucleotide Phosphodiesterase: Pronounced Stimulation by Snake Venom, Biochem Biophys Res Commun 29 , 478, 1967

Worthington Core enzyme ： Include ： Collagenase , Deoxyribonuclease I, Elastase , Papain , Ribonuclease , Trypsin, etc .