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BioVendor sRAGE Elisa试剂盒测试原理和注意事项
2022-06-30 20:08:00 【Sylvia_sc】
艾美捷 BioVendor RD191116200R sRAGE Elisa试剂盒是一种用于人sRAGE(晚期糖基化末端可溶性受体)的定量测定工具。

艾美捷 BioVendor sRAGE Elisa试剂盒测试原理:
在Biovendor Human sRAGE ELISA中,标准品、质量控制和样品均已孵育在微孔板中预涂有多克隆抗人sRAGE抗体。120分钟后孵育和洗涤,添加生物素标记的多克隆抗人sRAGE抗体与捕获的sRAGE孵育60分钟。再次清洗后,链霉亲和素HRP添加共轭。在30分钟孵育和最后一个洗涤步骤后,剩余的允许共轭物与底物溶液(TMB)反应。反应被测量添加酸性溶液和所得黄色产物的吸光度。这个吸光度与sRAGE的浓度成正比。标准曲线由绘制吸光度值与标准品的sRAGE浓度以及使用此标准曲线确定未知样品。
艾美捷 BioVendor sRAGE Elisa试剂盒注意事项:
− 仅供专业人士使用
− 处理免疫诊断材料时,应佩戴手套和实验室外套
− 请勿在处理免疫诊断材料的区域饮酒、进食或吸烟
− 该套件包含人类来源的组件。发现这些材料对HBsAg、HCV抗体和针对HIV的1/2抗原和抗体。但是,这些材料应被视为具有潜在传染性,因为任何测试都不能保证完全没有传染源
− 该套件包含动物源性成分。这些材料应按以下方式处理潜在传染性
− 避免接触含氢的酸性停止溶液和基底溶液过氧化物和四甲基联苯胺(TMB)。在以下情况下佩戴手套、眼睛和衣物防护处理这些试剂。停止和/或基质溶液可能导致皮肤/眼睛刺激。在里面如果接触停止溶液和基质溶液,请彻底清洗皮肤/眼睛如有必要,用水冲洗并就医
− 不得通过口吸管吸取材料
艾美捷 BioVendor sRAGE Elisa试剂盒相关文献:
1、Brett J, Schmidt AM, Yan SD, Zou YS, Weidman E, Pinsky D, Nowygrod R, Neeper M, Przysiecki C, Shaw A, et al. Survey of the distribution of a newly characterized receptor for advanced glycation end products in tissues. Am J Pathol. 1993 Dec;143 (6):1699-712
2、Emanuele E, D'Angelo A, Tomaino C, Binetti G, Ghidoni R, Politi P, Bernardi L, Maletta R, Bruni AC, Geroldi D. Circulating levels of soluble receptor for advanced glycation end products in Alzheimer disease and vascular dementia. Arch Neurol. 2005 Nov;62 (11):1734-6
3、Falcone C, Emanuele E, D'Angelo A, Buzzi MP, Belvito C, Cuccia M, Geroldi D. Plasma levels of soluble receptor for advanced glycation end products and coronary artery disease in nondiabetic men. Arterioscler Thromb Vasc Biol. 2005 May;25 (5):1032-7
艾美捷 BioVendor sRAGE Elisa试剂盒相关研究:
1、蛋白
RD172116100-HEK
Soluble Receptor for Advanced Glycation End Products (sRAGE) Human HEK293(HEK293表达的人)
RD172116100
Soluble Receptor for Advanced Glycation End Products Human E. coli(大肠杆菌表达的人)
RD272590100
Soluble Receptor for Advanced Glycation End Products (sRAGE) Mouse HEK293(HEK293表达小鼠)
2、抗体
RD184116100
Soluble Receptor for Advanced Glycation End Products (sRAGE) Human, Sheep Polyclonal
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