Explanation of history and chemical properties of Worthington ribonuclease B

AI Meijie Worthington  Ribonuclease B history ：

Jones is  1920  The work of 2004 is usually considered to be that of pancreatic ribonuclease “ start ”（Richards  and  Wycoff 1971）. Ribonuclease is produced by  Dubos  and  Thompson  On  1938  Year separation , And in  1940  Year by year  Kunitz  crystal .

1947  year ,Worthington  Become home to produce high-purity crystals  RNase  The company . stay  1950  In the early s ,Armor  The company prepared crude crystalline enzyme , And at a very affordable price . Whole  1960  Age and  1970  years ,RNase A  It has always been a hot research object , Mainly because it is very heat-resistant , And in high concentrations in available sources —— In bovine pancreas . These studies _ The way （Hantgan etc. . 1974）.RNase A  It is an enzyme and a third protein that determines the correct amino acid sequence （Raines 1998）.

And  RNase  Research related work has won four Nobel prizes （Anfinsen、Moore、Stein  and  Merrifield）. A lot of literature and research make  RNase  Become  20  The most widely studied enzyme in the th century （Raines 1998）.

Recent work continues to study the endoplasmic reticulum in living cells  RNase  Synthesis and maturation of （Geiger et al . 2010）. A lot of work is still devoted to research  RNase  Fold and gather （Benito wait forsomeone 2008、Iwaoka wait forsomeone 2008  and  Arai wait forsomeone 2010）. The role of this enzyme in cancer development and gene regulation is under study  (Shlyakhovenko 2009), And is being developed into a cancer chemotherapeutic agent  (Chao et al . 2010).

 

 

AI Meijie Worthington  Ribonuclease B Chemical properties of ：

Protein login number ： P61823

CATH  classification  (v. 3.2.0):

Category ：Alpha-Beta

framework ： rolling

Topology ：P-30  protein  

Molecular weight ：

RNase A：13.7 kDa (Hirs et al . 1956b)

RNase B：14.700 ± 0.3（Plummer  and  Hirs 1963）

The best  pH：RNase A：7.0-7.5（Brown  and  Todd 1955）

Isoelectric point ：

Ribonuclease  A：9.3（Ui 1971）

Extinction coefficient ：

RNase A  and  B：8,640 cm -1 M -1（ Theoretical value ）

RNase A：E 1%,280 = 7.3（Worthington RNase A）

RNase B：E 1%,280 = 5.77（ Theoretically ,RNase B）

Active site residues ：

Histidine （H12、H119）

Lysine  (K41)

Activator ：

sodium chloride  (Weickmann et al . 1981)

Sulfates （Moosavi-Movahedi wait forsomeone ,2006  year ）

Inhibitors ：

heavy metal ion

Ribonuclease inhibitor  (RI), A kind of  50 kDa  Protein , It accounts for the protein in the cytosol of mammalian cells ≤ 0.01% (Takahashi 1967)

Uridine - Vanadate complex  (Lindquist et al . 1973)