Worthington cytochrome c digestion study carboxypeptidase B scheme

Carboxypeptidase  B background ：

Carboxypeptidase  B (CPDB)  It is a metal carboxypeptidase , It catalyzes basic amino acids 、 Lysine 、 Arginine and ornithine from polypeptides  C  End position hydrolysis ：

Peptide group -L- Lysine (-L- Basic amino acids ) + H 2 O -->  peptide  + L- Lysine (L- Basic amino acids )

Carboxypeptidase is secreted by pancreatic acinar cells as an enzyme . Zymogen is activated by trypsin in the small intestine .

 

Worthington  Carboxypeptidase  B Specificity ：

CPDB  Activated by trypsin .CPDB  It has high specificity for lysine and arginine , But showed a preference for arginine （Tan  and  Eaton 1995）. It can also （ At a slower speed ） Acting on valine 、 Leucine 、 Isoleucine 、 Asparagine 、 Glycine and glutamine （Villegas wait forsomeone 1995,Nishihira wait forsomeone 1995）. And  CPDA  Medium  Gly207、Ile243  and  Ile255  comparison , Carboxypeptidase  A (CPDA)  and  CPDB  The specific difference between can be attributed to  CPDB  Medium  Ser205、Gly241  and  Asp253  Residue （Coll etc. , 1991）.

AI Meijie Worthington Carboxypeptidase  B works ：

CPDB  The main form of is monomer state .CPDB  Each mole of protein contains  1  Zinc atom . The residues coordinated with zinc residues are conservative , Including two histidine 、 A glutamic acid and a water  (Avilés and Vendrell 2004).

Worthington Carboxypeptidase  B Of 4 Applications ：

1. By continuous cutting  C  Sequence analysis of terminal basic amino acids

2. Serum markers for the diagnosis of acute pancreatitis （Avilés  and  Vendrell 2004）

3. Cytochrome  C  Gradually digest  (Südi et al. 1989)

4. Human insulin production （Ladisch  and  Kohlmann 1992）

Related literature ：

1.Akanuma, H.、Kasuga, A.、Akanuma, T.  and  Yamasaki, M.： Affinity chromatography is studying bovine carboxypeptidase  B  Effective application in complex formation with alkaline and aromatic acid analogues , Biochem Biophys Res Commun 45, 1971  year  2  month  27  Japan

2.Avrameas, S.  and  Uriel, J.： Systematic isolation of porcine pancreatic hydrolase . Two 、 stay  0.40  Fractionation of enzymes insoluble in ammonium sulfate solution at saturation , biochemistry  4 , 1750, 1965

3.Brunori, M.、Antonini, E.、Wyman, J.、Zito, R.、Taylor, J.  and  Rossi-Fanelli, A.： Study on redox potential of heme protein . Two 、 Carboxypeptidase digestion of human hemoglobin , J Biol Chem 239 , 2340, 1964