Lumiprobe bifunctional crosslinker sulfo cyanine 5 bis NHS ester

AI Meijie  Lumiprobe Sulfo-Cyanine5 bis-NHS  Ester is a water-soluble bifunctional dye , There are two  NHS  Ester function . It is  Cy5 bis-NHS  Analogues of esters .

The dye can be used for cross coupling of amines , And other advanced markup applications .

AI Meijie  Lumiprobe Sulfo-Cyanine5  double -NHS  ester chemical property ：

form ：  Dark blue powder

Molecular weight ： 975.14

Molecular formula ： C 45 H 51 KN 4 O 14 S 2

solubility ：  good  DMF、DMSO、 water

The quality control ： NMR 1 H, HPLC-MS (895%)

Storage conditions ：  Store ： stay  -20°C  Keep away from light . transport ： At room temperature for  3  Zhou . Avoid prolonged exposure to light . dry .

Spectral characteristics

Maximum excitation / absorption ,nm： 646

ε, L⋅mol -1 ⋅cm -1： 271000

Maximum emission , nanometer ： 662

Fluorescence quantum yield ： 0.28

CF 260： 0.04

CF 280： 0.04

quote ：

Pettersson, J.R.; Lanni, F.; Rule, G.S. Dual lifetimes for complexes between Glutathione-S-transferase (hGSTA1-1) and product-like ligands detected by single-molecule fluorescence imaging. Biochemistry, 2017, 56(31), 4073–4083. doi: 10.1021/acs.biochem.7b00030

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