Lumiprobe lumizol RNA extraction reagent solution

AI Meijie Lumiprobe Lumizol RNA  Extract reagent background ：

Lumizol The reagent contains phenol 、 Guanidine isothiocyanate and other components , Make high quality RNA Extraction becomes possible . Phenylphenol and guanidine isothiocyanate provide cell lysis and effective inhibition of ribonuclease . therefore  RNA  Be kept intact .

Phase separation occurs after the sample is homogenized and chloroform is added ： Upper aqueous phase 、 Interphase and lower organic phase . The water in the upper aqueous phase is then collected by precipitation with isopropanol  RNA, It is possible to collect from the interface phase and the organic phase  DNA  And protein . Ethanol solution can be used to separate from the mesophase and the lower organic phase  DNA, For proteins , This can be done by precipitation from the supernatant with isopropanol .

AI Meijie  Lumiprobe Lumizol RNA  Chemical properties of extraction reagent ：

form ： Yellow orange solution

The quality control ： A functional test

Storage conditions ： Store ： stay  +4°C  Keep away from the light  12  Months . transport ： At room temperature for  3  Zhou .

AI Meijie Lumiprobe Lumizol RNA  Extraction reagent Related research ：

Click on chemical buffer ,1.5x

Catalytic buffer for click chemical modification of nucleic acids and small molecules .

copper  (II)-TBTA  compound ,10 mM,55%  Aqueous solution . Dimethyl sulfoxide

Click Chemistry  Catalyst for biological conjugation synthesis .

DMF（ Dimethylformamide ）, Label level

be used for  NHS  Ester labeled dry high purity amine free solvent .

DMSO, Label level

Pure dimethyl sulfoxide  (DMSO)  solvent , Its quality is aimed at  Click  And other conjugated reactions

EDC, Coupling stage

Water soluble carbodiimide for coupling carboxylic acid with amine .

be used for  NA  Precipitated linear acrylamide

Nucleic acid coprecipitating agent . Apply to  PCR  and  RT-PCR  reaction .

THPTA  ligand

THPTA  It's a  Cu(I)  Water soluble ligands for catalytic click chemistry . The ligand stabilizes copper in its  Cu(I)  Oxidation state . Due to its high water solubility , The reaction with this ligand does not require organic solubilization