Deoxyribonuclease I instructions in Chinese and English

Bovine pancreatic deoxyribonuclease I produced recombinantly in yeast, Pichia pastoris, to decrease levels of contaminating RNase and eliminate potential pathogens associated with animal based materials.

Bovine pancreas is a rich source of RNase A which is often found in many commercial DNase preparations. Producing DNase I by recombinant means in an organism with much lower levels of endogenous RNase greatly facilitates purification of an enzyme with undetectable levels of RNase. The processes involved in the production and isolation of recombinant DNase I are completely devoid of animal based components which eliminates the possibility of introducing animal derived pathogens into bioprocessing procedures.

Recombinant DNase I is suitable for such applications as:

• Removing genomic DNA from RNA preparations prior to RT-PCR

• Degradation of DNA templates after transcription reactions

• Removing unwanted DNA from samples prior to Northern blotting

• Removing DNA during biopharma and bioprocessing procedures

Unit Definition: One Unit causes an increase in absorbance at 260nm of 0.001 per minute at 25oC when acting upon highly polymerized DNA at pH 5.0, which is the same as other Worthington DNase I products.

Note: Kunitz units as reported by other suppliers can be 2 to 4 times higher than Kunitz units as measured at Worthington. As measured at Worthington, One Kunitz unit digests 1mg of calf thymus (or pUC19 or l-phage) DNA in 10 minutes at 37oC in 50mM Tris, 1mM Mg2+, 1mM Ca2+, pH 7.8. Correlation of digestion units with Kunitz units may be different in other buffer systems.

Storage Buffer (DR1S): 5mM calcium acetate, 4mg/ml glycine, pH 5.0 and 50% glycerol.

DNase I Reaction Buffer (10X): 500mM Tris-HCl, 10mM MgSO4, 1mM CaCl2, pH 7.8, provided.

AI Meijie Worthington Deoxyribonuclease I Specificity ：

bpDNase I  Not base or sequence specific ; however , It does not cut randomly . It shows priority in pyrimidine  5'  Side cracking , And especially in alternative copolymers （Bernardi  wait forsomeone  1975  and  Lomonossoff  wait forsomeone  1981）. It has been shown that , The change of twist angle is  DNase I  distinguish （Dickerson  and  Drew 1981）.DNase I  The specificity also depends on the presence of divalent cations . In existence  Ca2+  and  Mg2+  Under the circumstances , It can cause single chain breaks , But in being  Mn2+  It will cause double chain breakage （Junowicz  and  Spencer 1973, as well as  Campbell  and  Jackson 1980）.

Deoxyribonuclease I Related research ：

Actin

Albumin , Nuclease free

Deoxyribonuclease  II

Deoxyribonucleic acid and related products

Histone

Lysozyme

Nuclease , Micrococcus

Nuclease ,S1

Phosphatase , alkalinity

Phosphodiesterase  I

Phosphodiesterase  II

protease K

Reverse transcriptase , restructuring  HIV

Ribonuclease A

Ribonuclease  T1

Ribonuclease ,E-Rase RNase  Mixture

RNA