Fluorescence imaging of cle19 polypeptide in cells preparation of fluorescence quenching quantum dots of bovine serum albumin

The scientific research content that Xiaobian will share with you today is cells CLE19 Peptide fluorescence imaging bovine serum albumin fluorescence quenching quantum dots The preparation of , Watch it with Xiaobian ！

cells CLE19 Peptide fluorescence imaging bovine serum albumin fluorescence quenching quantum dots preparation ：

Quantum dots (QDs) And precious metals (Au,Ag) Nanoclusters (NCs) As a new fluorescent nano material , In chemical testing , Biosensors and other fields have been widely used . However ,QDs And precious metals (Au,Ag) NCs There are few applications in plant cell marker imaging . Based on this , To build the CdTe QDs And precious metals (Au,Ag)NCs Functionalized fluorescent probe .

The main research contents are as follows ：

1. Synthetic glutathione (GSH) Embellished CdTe QDs, We adopt EDC/NHS Covalent catalysis and direct adsorption methods compose the sequence as NH2(CH2)6RVIPTGPNPLHN( abbreviation P1) and CCYRVIPTGPNPLHN( abbreviation P2) Of CLE19 Polypeptides and GSH-CdTe QDs coupling , Prepare two GSH-CdTe QDs Fluorescent probe , Using fluorescence spectrum , Ultraviolet - Visible absorption spectrum , Resonance light scattering spectroscopy is used to characterize it . Columbia type Arabidopsis plants were planted and Arabidopsis protoplasts were prepared by enzymatic hydrolysis , Make it functional GSH-CdTe QDs Fluorescent probes are used on the cell surface of Arabidopsis protoplasts CLE19 Imaging of peptide hormone receptors . 

2. With P2 Functionalization for template in situ synthesis Au NCs and Ag NCs, Using fluorescence spectrum , Ultraviolet - Visible absorption spectrum , It was characterized by dynamic light scattering particle size analyzer , The results show that the synthesized Au NCs and Ag NCs Both emit green fluorescence . take Au NCs Blend culture with Arabidopsis protoplast cells and used for CLE19 Imaging of peptide hormone receptors . 

3. Synthetic histidine modified Au NCs, Using fluorescence spectrum , Ultraviolet - Visible absorption spectrum , Circular dichroism spectrum , Raman spectroscopy and other means have studied Au NCs With bovine serum albumin (BSA) Interaction of .

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Ruixi YWX.2022.7.27