Yongzhou plant cell laboratory construction layout plan

What should be considered in the construction of Yongzhou Plant Cell Laboratory ？SICOLAB Xiaobian will take you to talk about construction knowledge from this article

One 、 Plant cells To cultivate

Under sterile and artificially controlled conditions , Plant organs in vitro 、 organization 、 cells , Culture on artificially prepared medium , Give appropriate cultivation conditions , Induce it to produce callus 、 Clump bud , Finally, a complete plant is formed .

Two 、 Practical application of plant cell engineering

1、 New ways of plant reproduction

Micropropagation ： It can efficiently and quickly achieve mass reproduction of seedlings

Crop detoxification ： Shoot tip tissue culture was used to remove the virus and obtain virus-free seedlings （ Because near the plant meristem （ Such as stem tip ） There is little or no virus ）

Artificial seeds ： Embryoids obtained from plant tissue culture 、 Adventitious buds 、 Apical buds and axillary buds are used as materials , Seeds packaged with artificial film . advantage ： Completely maintain the genetic characteristics of good varieties , Not limited by season ; Convenient for storage and transportation

The structure of artificial seeds includes ： Artificial seed coat 、 Embryoids and artificial endosperm .

2、 Cultivation of new crop varieties

（1） Haploid breeding ：

a The process ： plant （AaBb） Pollen is obtained by meiosis （AB、Ab、aB、ab Four types ）; Pollen was cultured in vitro （ Technology is plant tissue culture ）; Get haploid plants ; Colchicine treatment was carried out at the seedling stage ; Normal homozygous diploid plants were obtained （AABB、AAbb、aaBB、aabb Four types ）.

b advantage ： Significantly shorten the breeding period

（2） Utilization of mutants

In the process of plant tissue culture , Because the cultured cells have been in a continuous meristematic state , Therefore, it is prone to mutation under the influence of culture conditions and external pressure , From these mutated individuals, we can screen out mutants that are useful for people , And then cultivate new varieties .

Plant cell laboratory Construction plan

1. Design principles

science 、 Efficient 、 Economical and practical .

2. Key points of site selection

quiet 、 clean 、 Away from pollution sources

3. Basic composition

Preparation room 、 Washing and sterilization room 、 Sterile operation room 、 Culture room 、 Buffer room , It is the basic condition for tissue culture experiment .

4. Equipment list

Ultraviolet lamp 、 Air conditioner 、 Anatomic microscope 、 Sterilizer 、 Alcohol lamp 、 Inoculation equipment （ Inoculation forceps 、 scissors 、 Scalpel 、 Inoculation needle ）, The experimental station 、 And shelves , There is also an ultra clean workbench and a complete set of sterilization and inoculation instruments 、 Medicine, etc .