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Lumiprobe蛋白质定量丨QuDye 蛋白定量试剂盒
2022-06-30 20:08:00 【Sylvia_sc】
艾美捷 Lumiprobe QuDye 蛋白定量试剂盒背景:
该试剂盒包括浓缩的 200x 测定试剂、稀释缓冲液和针对测量领域优化的 0、200 和 400 ng/uL 蛋白质标准品。根据蛋白质类型的不同,该测定具有低变异性,并且可以耐受污染物,如还原剂、核苷酸、游离氨基酸 DNA,但不能耐受去污剂。该试剂盒允许在 12.5 µg/mL 至 5 mg/mL 范围内对初始样品进行准确的蛋白质定量(使用荧光计测量的蛋白质量范围:200 µL 测试样品中的蛋白质含量为 0.25-5 µg)。实验在室温下进行,信号稳定3小时。
当使用相同的缓冲液来计算背景和制备样品时,测量的质量甚至会更高。
艾美捷 Lumiprobe QuDye 蛋白定量试剂盒允许简单地稀释试剂浓缩物并测量。荧光计仪器允许读取体积为 1–20 uL 的样品。

艾美捷 Lumiprobe QuDye 蛋白定量试剂盒相关研究:
ProteOrange 蛋白质定量试剂盒
该蛋白质定量试剂盒的核心是一种非常灵敏的 ProteOrange 荧光染料。该染料适用于荧光分光光度计和酶标仪。该试剂盒针对蛋白质定量进行了优化,最终溶液中蛋白质的检测水平低至 10 ng/mL。
ProteOrange 蛋白质定量试剂,500x
灵敏的荧光检测试剂 500x 浓缩液,用于定量纯化的蛋白质样品。该试剂显示出不同蛋白质的最小变异性。
QuDye 蛋白试剂,200x
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