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find_ Detailed use guide of CIRC

2022-06-27 00:22:00 Black faced boy

First fine_circ Is in py27 function , need numpy and pysam These two libraries ,pysam I won't support it windows System , So it is recommended that linux Run under . Use the software VMWare,pycharm.

1 pysam install

Try first pip install pysam perhaps conda Instructions , If you fail , Try adding the image file once , If it still fails , Please use wheel Files installed .

2 bowtie2 and Samtools install

Refer to the following links :

Web of Science —Bowtie2 and Samtools Software update - Lu Rui's blog

3 Circ_find Use

(1)bowtie2 Generate genome index

use first bowtie2 Generate .bam Sort files , Other comparison programs can also , Can produce bam All files are OK

(2)

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