Worthington - chemical properties and related studies of Worthington trypsin

Trypsin is a pancreatic serine protease , It has substrate specificity based on positively charged lysine and arginine side chains . It comes from  34 kDa  Inactive precursor zymogen trypsinogen , In enzymatic removal  N  End  6  After the amino acid precursor sequence  23.8 kDa  Trypsin molecule . Most suitable pH by 8.0. Trypsin is replaced by organophosphorus compounds （ Such as diisopropyl fluorophosphate ） And natural inhibitors from the pancreas . soybean 、 Lima beans and egg white are also sources of natural inhibitors . Trypsin cleavage  Arg  and  Lys  Amide bond and ester bond of .

AI Meijie Worthington  Sequencing grade trypsin has been further purified , In order to remove traces of contaminated protease and autolytic products that may interfere with trypsin digestion experiments , And in  PAGE  A single strip is displayed on .

 

 

AI Meijie Worthington Chemical properties of trypsin ：

purpose ： For tissue culture ,Worthington  Trypsin , Code ：TRL、TRLS、TRLVMF  and  TRTVMF  It has been used by many researchers .

Code ：TRSEQZ、TRSEQII  and  TRTPCK  Usually used for protein sequencing 、 Mapping and structural research .

Worthington  Improved sequencing grade trypsin ,  SequENZ, Product code ：TRSEQZ, It is widely purified to remove contaminated proteases and trypsin autolysis by-products that may affect the specificity of the digestion process . And then , Chemically modify the enzyme , To minimize the autolysis process and increase stability . The modified trypsin is further processed to remove the residual self degradation products . After chemical modification, the specificity of the enzyme is routinely checked .

stability / Store ： Most grades  Worthington  Trypsin in  2-8°C  Stable when stored  2-3  year . Moisture-proof .

Unit definition ： TAME  Company ： A unit in  25°C、pH 8.2  and  10 mM  Hydrolyze every minute in the presence of calcium  1 µmole P-toluenesulfonyl -L- Methyl arginine  (TAME).  

Be careful ：1mg  Trypsin ≥180 TAME  Company 、10,350 BAEE  Company 、3,450 USP/NF  Company .

Technical specification ：  Virus free and Mycoplasma trypsin （ Code ：TRTVMF） Passed  0.22  Membrane filtration with micron pore size , Freeze drying , Be exposed to gamma rays , And test for viruses and Mycoplasma .

AI Meijie Worthington Research on trypsin ：

Chymotrypsin  (CDAG/CDS/CDI)

Collagenase  (CLS1-4/CLSPA)

Deoxyribonuclease  I (DP/D/DCLS/D2/DPFF/DPRF)

Hyaluronidase  (HSE/HSEP)

Neutral protease （ Dispersing enzyme ,NPRO）

protease  K (PROK)

Trypsin inhibitor  (LBI/OI/SI/SIC)

Cell separation optimization system  (CIT)

Hepatocyte isolation system  (HIS)

Neonatal cardiomyocyte isolation system  (NCIS)

Papain dissociation system  (PDS/PDS2)