Operating instructions for abbexa AEC chromogen Kit

AI Meijie  Abbexa AEC  The chromogen kit contains  AEC（3-Amino-9-Ethylcarbazole）, A chromogen widely used in immunohistochemical staining .AEC  It is the substrate of peroxidase , Produce a red product soluble in alcohol , It must be used with water-based re dyeing agent and sealing agent . It produces clean 、 A strong stain , There is no unnecessary background .

AI Meijie AbbexaAEC  Chromogen kit kit components ：

PBS：2×30 ml

Substrate reagent ：3 ml

AEC Chromogenic agent ：3 ml

AI Meijie Abbexa AEC  Instructions for use of chromogen kit ：

1. Not long before incubation , preparation  AEC  Substrate reagent , The method is to add... In a clean test tube  1 ml PBS、50 µl  Substrate reagents and  50 µl AEC  Chromogen . Mix well .

2. Smear on each slide  50-100 µl  Work  AEC  Substrate reagent . Incubate  5-10  minute .

3. After full staining , stay  PBS  Wash the glass slide in  3  minute . Repeat this washing step for a total of  3  Time .

notes ：

1. Do not use alcohol solution for re dyeing , because  AEC  Dyes are soluble in organic solvents .

2. Nitrocellulose strips usually have a slight red background .

3. When testing , Use personal protective equipment  (PPE), For example, lab coat 、 Gloves and goggles .

AI Meijie Abbexa  AEC  Chromogen kit related articles ：

1、 Putative cancer stem cell markers  CD117  and  CD15  Expression and prognostic value in choroidal and ciliary melanoma .

Lukenda A,Dotlic S,Vukojevic N,Saric B,Vranic S,Zarkovic K.

Journal of clinical pathology .2016  year  3  month ;69(3):234-9.doi：10.1136/jclinpath-2015-203130. The electronic  2015  year  8  month  19  Japan .

PMID: 26290260

result ： When using AEC  Chromogen when , stay  31  Of the samples  12  individual  (39%)  Observed in  CD117  Overexpression , But in the use of  DAB  when , stay  26  Of the samples  14  individual  (54%)  Observed in  CD117  Overexpression .

2、 Transpupillary thermotherapy with short time laser irradiation  (TTT)  Heat shock proteins are induced in the chorioretinal layer  (HSP)  High expression

Desmettre T, Maurage CA, Mordon S.

Laser surgery medicine .2003;33(2):102-7.doi: 10.1002/lsm.10193.

PMID: 12913881

result ：  During the experiment , Except for the strongest of each exposure duration “ high ” Outside power , The laser spot is not visible , Whitening can be identified at the end of laser exposure . In order to “ moderate ” The laser power is continuously  60、30  or  15  Seconds of laser irradiation , A strong  HSP70  Immune reactivity . contrary , It's going on  60、30  or  15  Of a second “ high ” or “ low ” Rare... Detected at laser power  HSP  Over expression . It was not detected outside the irradiation area of the control eye and the treatment eye  HSP-70  Immune reactivity .