Operation method of abbkine elikine human alpha fetoprotein (AFP) ELISA quantitative Kit

Alpha fetoprotein （AFP） It is a highly specific and sensitive tumor marker of primary liver cancer . its  Cutoff  The value is usually in 20 μg/L, When more than 500 ug/L Or when the content continues to rise , It has definite significance .

Experimental suggestions ：

EliKine Human alpha fetoprotein （AFP）ELISA The quantitative kit uses double antibody sandwich method to quantify human alpha fetoprotein in the sample （AFP）. Anti human alpha fetoprotein （AFP） The specific antibody is coated on the enzyme label plate , Add the sample or standard into the hole of the enzyme label plate , Human alpha fetoprotein （AFP） It will bind to the solid-phase antibody coated on the plate . Unbound ingredients are washed away , And then HRP Coupled anti human alpha fetoprotein （AFP） Specific antibody is added into the hole . Remove unbound HRP  After antibody coupling , Add chromogenic substrate （TMB）,TMB  stay  HRP  Under catalysis, it appears blue , It turns yellow after adding termination liquid . Use a microplate reader in  450 nm  Wavelength measurement OD  value , Human alpha fetoprotein （AFP） Concentration and  OD450  Values are proportional .

Human alpha fetoprotein （AFP）ELISA Quantitative kit components ：

• Human AFP Microplate

• Human AFP Standard (lyophilized)

• Sample Diluent (5×)

• Assay Buffer (5×)

• HRP-conjugated Human AFP Detect Antibody (100×)

• HRP Substrate（TMB）

• Stop Solution

• Wash Buffer (20×)

• Plate Covers

EliKine Human alpha fetoprotein （AFP）ELISA Advantages of quantitative kit ：

Its detection has high sensitivity and excellent specificity , No association with human alpha fetoprotein was observed （AFP） Significant cross reactions or interferences between analogues .

Precautions in use ：

1.  If  Sample Diluent (5×) and  Assay Buffer (5×) The color turns yellow , A small amount of precipitation and other phenomena , It is because the reagent contains serum , Centrifuge to remove the sediment , Does not affect normal use .

2.  Different batch numbers 、 Do not mix components from different manufacturers ; otherwise , May cause abnormal results .

3.  Change suction head frequently , Avoid cross contamination between components .

4.  To ensure accurate results , The plate must be adhered correctly during the incubation step .

5. Stop Solution  It is corrosive , Please take protective measures during operation .