Storage and steps of phospholipid coupled antibody / protein Kit

Phospholipid coupling kit can be used by antibody / The primary amine group on the protein （ Such as lysine ）, Simple 、 Quickly realize antibody / Covalent coupling of protein and phospholipids . This is a general-purpose kit , Suitable for antibodies of various molecular weights / Protein is coupled with phospholipids . The whole coupling process is 2 h Finish in , The required manual operation time is about 3 minute . Xiaobian will take you to understand Phospholipid coupled antibody / Protein kit Storage and preparation of ！

Kit composition and storage

Store phospholipids in -20⁰C. Phospholipid solution 、 The reaction buffer can be used in +4°C Short term storage or -20°C Long term preservation .

The experimental steps

（ Coupled 1mg antibody / Take protein as an example ）

1、 take 1~2mg Phospholipids are dissolved in 50μL Phospholipid solution , take 1mg antibody / Protein dissolved in 1mL In the reaction buffer .

2、 take 50μL Phospholipid solution and 1mL antibody / After the protein is mixed evenly , React at room temperature 2h.

3、 After the reaction , Transfer the reaction solution to the ultrafiltration tube , With 9500rpm,4°C Centrifugal ultrafiltration removes all solvents , Wash with pure water 2 Times to remove unreacted phospholipids .

4、 Phospholipid coupled antibody / Protein storage ： Phospholipid coupled antibody / Post protein , We suggest storing the conjugate in +4°C. The overall stability of the conjugate will be determined by the antibody / The stability of the protein itself determines , Because it will degrade first . If your antibody / Antibody stability , The conjugate will also be stable .

matters needing attention

The kit can be compatible with amine free buffer （PBS,MES,MOPS, HEPES） and <50% glycerol . If the sample antibody / Protein contains the following components , May interfere with the sample antibody / Coupling of protein and phospholipids .

Ruixi YWX.2022.7.28