CpG solid support research: lumiprobe general CpG type II

AI Meijie Lumiprobe Universal CPG type II, 1000A  It is a universal support , It is used to immobilize nucleosides to synthesize oligonucleotides and improve the efficiency in the process of deblocking  3'  Dephosphorylation rate of terminal oligonucleotides .

For cleavage from the carrier and oligonucleotide deprotection of the anhydrous ammonia gas phase , Ammonium hydroxide can be used in a short time / Methylamine mixtures and other basic reagents .Universal CPG type II, 1000A  Suitable for use in harsh conditions , Compared with general carrier , Faster cutting and deprotection .1000 Å  The pore size of is recommended for synthesis up to  120  A base oligonucleotide .

AI Meijie  Lumiprobe Universal  CPG II  type , 1000A usage ：

usage

coupling ： Universal  CPG  Standard conditions for .

Deprotection ： stay  80 °C  Next  2  Hours or at  55 °C  Use concentrated ammonia water  8  Hours ; Use  AMA  Mixture 、 Ammonium hydroxide  - 40%  Methylamine  (1:1)  stay  65 °C  Next  15  minute .

AI Meijie Lumiprobe Universal  CPG II  type , 1000A Related research ：

Universal  CPG I  type ,500A

This universal support is designed for automatic synthesis under harsh unclosed conditions and extended cutting times  3'  Terminal modified oligonucleotides . recommend  500 Å  The aperture of is used to synthesize a length not exceeding  50  A base oligonucleotide .

TFA- Amino modifier  CPG 500

Used in the synthesis of oligonucleotides  TFA- Amino modifier  CPG.500 Å, High load controllable hole glass .

Phosphate  CPG 500  Solid carrier

For synthesis  3'- Phosphorylated oligonucleotide  CPG  Solid support .

Phosphate  CPG 1000  Solid carrier

Phosphate modifier  CPG, For solid-phase synthesis containing  3  Phosphate modified long （ Most  120  Polymer ） Oligonucleotides .

FAM CPG 500,6  Isomer

Used to synthesize compounds containing  3'- fluorescein  (FAM)  Modified controllable pore glass of modified oligonucleotides .

DusQ3 CPG 500

DusQ3  They are far red and near infrared dyes （ Such as  Cyanine5.5） Dark quencher for . This is a controllable porous glass solid support , For the synthesis of oligonucleotides , Quencher connected to  3'- end .

DusQ2 CPG 500

Controllable hole glass  (CPG) 500  Improved  3'  End with  DusQ2  The oligonucleotide synthesis of quencher supports . Compatible with standard deblocking conditions .

DusQ2 CPG 1000

Controllable hole glass  (CPG) 1000  Improved  3'  With  DusQ2  The oligonucleotide synthesis of quencher supports . Compatible with standard deblocking conditions .