Reactive dye research: lumiprobe af594 NHS ester, 5-isomer

AF594  It is a bright water-soluble dye , Yes  4  To  10  Within the scope of  pH  Change insensitive . This red fluorescent dye is usually used for flow cytometry and fluorescence microscopy .

AI Meijie Lumiprobe AF594 NHS  Esters are used to label proteins 、 peptide 、 Antibodies and anything that contains  -NH2  Molecules of groups （ Such as amino modified oligonucleotides ）; It leads to the formation of stable amide bonds between the dye and the target molecule . stay  7  To  9  Of  pH  The best result of the binding reaction is achieved in the range of .

AF594  It can be used for protein labeling with high dye to protein molar ratio . The resulting has a height marker  (DOL)  The conjugate of showed no significant fluorescence quenching . by comparison , The conjugates have brighter fluorescence , This can increase the minimum detection limit of the labeled product .

AI Meijie  Lumiprobe AF594 NHS  ester ,5- Chemical properties of isomers ：

form ：  Dark blue crystal

Molecular weight ：1022.23

CAS Number ： 1638544-48-5

Molecular formula ：C 51 H 67 N 5 O 13 S 2

IUPAC  name ：5-(((2,5- Dioxypyrrolidine -1- The base ) Oxy ) carbonyl )-2-(1,2,2,10,10,11-hexamethyl-4,8-bis(sulfonatomethyl)-10,11- dihydro-2H-pyrano[3,2-g:5,6-g']diquinolin-1-ium-6-yl)benzoate

solubility ： dissolve in water 、DMSO、DMF

The quality control ：NMR 1 H, HPLC-MS (95%)

Storage conditions ： Store ： stay  -20°C  Keep away from light . transport ： At room temperature for  3  Zhou . Avoid prolonged exposure to light . dry .

AI Meijie Lumiprobe AF594 NHS  ester ,5- different Construct Spectral characteristics ：

The greatest inspiration / absorption ,nm： 590

ε, L⋅mol -1 ⋅cm -1： 110000

The largest launch , nanometer ： 617

Fluorescence quantum yield ： 0.99

CF 260： 0.42

CF 280： 0.60

AI Meijie Lumiprobe AF594 NHS  ester ,5- Isomer Related research ：

AF488 NHS  ester

AF488 NHS  ester , A bright for green channels 、 Photostability and hydrophilic fluorescent dyes .

AF568 NHS  ester

AF568  derivatives , Used for labeling proteins and other high and low molecular weight ligands with free amines .

AF594 NHS  ester ,5- Isomer

AF594  The red fluorescence of the dye  NHS- ester .

AF647 NHS  ester

Bright water soluble succinimide ester  AF647, Used to label proteins and antibodies .

BDP 558/568 NHS  ester

BDP 558/568  It is a fluorescent group in the Yellow Region of the spectrum . And others  BDP  Like dye , This fluorophore has a high fluorescence quantum yield 、 Brightness and light stability .

BDP 576/589 NHS  ester

BDP 576/589 NHS  ester （BODIPY 576/589  Analogues ） It is a bright hydrophobic dye , Emitting in the orange spectral range . It can be used to measure the fluorescence anisotropy .

BDP 581/591 NHS  ester

BDP 581/591  Of boron Dipyrrole methylene fluorophore  NHS  Ester derivatives , Used to label proteins 、 Peptides and other biomolecules . The dye can be used in fluorescence polarization and two-photon experiments , And can be used as  ROS  probe .

 

BDP 630/650 X NHS  ester

BDP 630/650  It is a boron Dipyrrole methylene fluorophore , Tune to  Cyanine5  Excitation and emission channels . The dye is bright and highly photostable . It can be used for fluorescence polarization measurement This is an amine reactivity  NHS  Ester derivatives .