Abnova liquidcell negative enrichment cell separation and recovery system

AI Meijie Abnova LiquidCell It is a non-invasive negative enrichment system , Used to consume white blood cells  (WBC)  And counting and recycling rare cells  (CRC). In translational and clinical studies  CRC  The three main subtypes of are circulating cells  (CTC)、 Circulating progenitor cells  (CPC)  And circulating fetal cells  (CFC). The market adopts  CRC  The challenge is high purity and feasibility  CRC  Efficient and repeatable identification of 、 Single cell isolation and recovery , Its application has been widely standardized  GMP  Support of biological reagents .

LiquidCell Platform utilization  AbBeads、SuperSlide、tyramide TYRx And color rendering  CHRx Technical realization  CRC  Separation and detection .AbBeads It is used for white blood cells  (WBC)  Consumed antibody functionalized beads , And avoid the accompanying  CRC  Loss .SuperSlide It is a kind of non autofluorescence glass slide with special coating , It can be captured and retained to the maximum extent during the whole process of cell treatment  CRC.TYRx Using tyramine fluorescence and horseradish peroxidase  (HRP)  Coupling secondary antibody enhances  CRC  The signal of low density protein .CHRx Use  DAB  and  Fast Red  Chromogenic substrate , Binding horseradish peroxidase  (HRP)  And alkaline phosphatase  (AP), In visible light  CRC  Stain brown and red .

Monoclonal antibodies against intracellular and cell surface proteins can be interchanged flexibly , To adapt to biomarker conversion in disease progression . This leads to downstream protein characterization 、 High purity and feasibility of gene analysis and cell assay  CRC.CTC  The count is now  FDA  Approved only 1 As a metastatic breast cancer 、 Prognostic markers for prostate cancer and colorectal cancer . by comparison , Effective  CRC  Single cell isolation and repair will open up new clinical applications in the field of diagnosis and pharmacy . 

CRC The subtype of

circulating tumor cell  (CTC) Circulating progenitor cells  (CPC) Circulating fetal cells  (CFC)

CRC Application ：

1、 Protein characterization

Immunofluorescence  (IF) Immunocytochemistry  (ICC)

2、 Gene analysis

Fluorescence in situ hybridization  (FISH) Polymerase chain reaction  (PCR)

Gene amplification  (GA)

Somatic mutations  (SM)

Single nucleotide polymorphism  (SNP) Comparative genomic hybridization  (CGH)

Copy number variants  (CNV) Next generation sequencing  (NGS)

3、 Cell detection

AI Meijie Abnova LiquidCell Platform advantages ：

WBC  Non destructive and non aggregative removal

WBC  The concomitant cell loss during depletion zui Small

Maximum cell recovery for downstream identification

Low density biomarker zui Big signal detection

AI Meijie Abnova  Studies on fluorescence detection of antibodies ：

ASGPR1  Monoclonal antibodies  (FITC)

CD133  Monoclonal antibodies  (APC)

CD44  Monoclonal antibodies  (APC)

CD44v9  Monoclonal antibodies

CD276  Monoclonal antibodies  (Alexa 647)

CD276  Monoclonal antibodies  (APC)

CD45  Monoclonal antibodies  (Alexa 647)

CD45  Monoclonal antibodies  (PE)

CD45  Monoclonal antibodies  PLUS (PE)

CDX2  Monoclonal antibodies  (FITC)

Cell surface vimentin  (CSV)  Monoclonal antibodies  (Alexa 488)

Cell surface vimentin  (CSV)  Monoclonal antibodies  (APC)

Cell surface vimentin  (CSV)  Monoclonal antibodies  (FITC)

Cell surface vimentin  (CSV)  Monoclonal antibodies  (PE)

Disasialic ganglioside  GD2  Monoclonal antibodies  (Alexa 647)

EGFR  Monoclonal antibodies

EpCAM  Monoclonal antibodies  (Alexa 488)