Lumiprobe non fluorescent alkyne research - dbco NHS ester

AI Meijie Lumiprobe DBCO NHS  Ester background ：

Dibenzocyclooctyne  (ADIBO, DBCO)  It is the corresponding strain that promotes the cycloaddition of alkyne azide  (SPAAC)  One of the most reactive cycloalkynes  -  A copper free click chemical reaction .

This is an amine reactivity  NHS  ester , The reactive moiety can be easily attached to almost any primary or secondary amine group , For example, protein 、 A group of peptides or small amines .

DBCO  Immediately react with azide . The reaction rate is much higher than that of copper catalyzed reaction , And reactions with many other cyclooctynes . Unlike some other cyclooctynes ,DBCO  Does not react with tetrazine  -  This allows azides to interact with  DBCO  And the orthogonal conjugation of trans cyclooctene and tetrazine .

AI Meijie  Lumiprobe DBCO NHS  ester chemical property ：

form ： Grayish white solid

Mass spectrometry  M+  The incremental ： 315.1

Molecular weight ：430.45

CAS Number ： 1384870-47-6

Molecular formula ：C 25 H 22 N 2 O 5

IUPAC  name ：

6-{2- Azatricyclic [10.4.0.04,9]hexadeca-1(16),4,6,8,12,14-hexaen-10-yn-2-yl}-6-oxohexanamide

solubility ： Good at  DCM、DMF、DMSO

The quality control ：NMR 1 H, HPLC-MS (95%)

Storage conditions ： Store ： stay  -20°C  Keep away from light . transport ： At room temperature for  3  Zhou . Avoid prolonged exposure to light . dry .

AI Meijie Lumiprobe DBCO NHS  Ester related research ：

Alkyne maleimide

Used to attach alkyne functional groups to proteins 、 Peptides and others with  SH（ mercaptan ） Groups （ Including cysteine residues ） On the molecule of maleimide reagent .

Alkyne  NHS  ester （ Hexylic acid  NHS  ester ）

Alkyne  NHS  ester , Used for labeling biomolecules with alkyne groups , Click chemistry for copper catalysis .

DBCO NHS  ester

For strain promoting copper free click chemistry  (SPAAC)  Azodibenzocyclooctyne  (DBCO, ADIBO)  Reagents . The reagent contains  NHS  Ester function , Used to attach cyclooctynes to various molecules .

Alkyne hydrazide

Bifunctional reagents containing alkynyl and hydrazide functional groups . It reacts with aldehydes and ketones . Then the alkynyl group can pass through CuAAC The reaction is coupled with various azides .

DBCO STP  ester

Used to modify proteins / antibody / Active ester of peptide , Then the copper free click chemical reaction is introduced .

EdU（5- Ethynyl -2'- Deoxyuridine ）

EdU  It's a nucleoside , Reproducible by incorporation of cellular enzymes  DNA  in . after , Contained in cells  DNA  It can be obtained by mixing with the fluorescent dye azide  Click  Chemical reaction for color development , To reveal cell proliferation .

EU（5- Ethynyluridine ）

Uridine alkyne derivatives are used to monitor and study  RNA  Transcription .EU  Labeled cells  RNA  With fluorescent dyes or biotin azides  CuAAC  Reaction and subsequent fluorescence measurements .

Lumiprobe Corporation Devoted to the study of ： Reactive dyes （ReactiveDye） and SYBRGreen I dyestuff , Customized synthetic fluorescent double labeled primers . EMG technology is Lumiprobe Corporation Our Chinese agent , Provide high-quality products and services for scientific researchers .