Discussion on the construction of Fuzhou nucleic acid testing laboratory

What are the instructions for the construction of Fuzhou nucleic acid testing laboratory ？SICOLAB Xiaobian will talk with you about construction issues from multiple perspectives

One 、 Nucleic acid testing laboratory Build internal structure

The laboratory is divided into decontamination areas 、 Sample acceptance area 、 Changing area 、 Reagent preparation area 、 Sample handling area 、 Amplification region and amplification product analysis region , And there are special corridors , Reagent preparation area 、 Sample handling area 、 Buffer room shall be set in four separate working areas of amplification area and amplification product analysis area , Interlocking devices should be installed between the working area and the buffer room . Work areas with different functions shall be separated and independent , There are obvious signs in each working area , No direct connection , If tightly connected , It is necessary to install an article transfer window , Do not reverse flow . The gas flow in the laboratory should also flow from the pre amplification area to the post amplification area , Do not reverse flow . UV lamps should be installed at the top of each work area , The wavelength of the ultraviolet lamp is 254mm, Every time 20 m One 40W The ultraviolet lamp .

Two 、 Nucleic acid testing laboratory Construction details

Decontamination area : Place cleaning equipment , Clean the experimental equipment, etc .

Sample acceptance area : Receipt and acceptance of samples .

Changing area : Change work clothes .

Reagent preparation area : Preparation of reagents 、 Repacking and preservation .

Sample handling area : Sample registration 、 Subpackage ; Nucleic acid extraction 、 preservation .

Amplification region : Preparation of amplification reaction mixture 、 Sample addition .

Amplified product analysis area : Determination of amplification products 、 Result analysis 、 Registration and reporting .

The pre amplification region and post amplification region should be strictly separated , Different rooms must be used , There is a certain interval between the two areas .

3、 ... and 、 Nucleic acid testing laboratory Building ： Configuration list

Nucleic acid extractor (96 hole )、-86°C Low temperature refrigerator 、 Biosafety cabinet 、 Electric constant temperature incubator 、 Automatic fluorescence quantification PCR Instrument 、 Color laser printers 、 Imported single channel pipette ( With support )、 Pure water machine 、 Super clean table 、 Medical dustbin 、 Hand disinfectant, etc