Classification and determination method of Worthington stemxyme

AI Meijie Worthington STEMxyme background ：

STEMxyme 1 & 2  Collagenase / Neutral protease  (Dispase)  It is a Clostridium histolyticus collagenase of no animal origin （ Code ：CLSAFB） And neutral protease of Bacillus polymyxa without animal origin  (Dispase)  Special combination of , At least  250  individual  CLS  Units and  1,000  Each milligram of dry weight contains  2,000  Casein units . Designed for stem cells 、 Regenerative medicine and other primary cell separation and bioprocessing applications , In these applications, it is necessary to prevent the introduction of potential pathogens of animal origin .

Worthington  A detailed description of collagenase and contaminant detection can be found in Worthington  Found in the enzyme manual . Besides , Tissue specific references and detailed separation conditions can be found in  Worthington  Found in the organization separation guide .

 

 

AI Meijie Worthington STEMxyme Classification description of ：

1.STEMxyme 1, Collagenase / Neutral protease （ Dispersing enzyme ）

source ：  Clostridium histolyticum / Bacillus polymyxa

A special collagenase of Clostridium histolyticus without animals （ Code ：CLSAFB） Combined with neutral protease of Bacillus polymyxa without animals , At least it contains  250  individual  CLS  Units and  1,000  Casein units per milligram dry weight . Designed for stem cell and other primary cell separation and bioprocessing applications , In these applications, it is necessary to prevent the introduction of potential pathogens of animal origin .

Stored in the  2-8°C.

2.STEMxyme 2  Collagenase / Neutral protease （ Dispersing enzyme ）

source ：  Clostridium histolyticum / Bacillus polymyxa, Clostridium histolyticus without animals Collagenase （ Code ：CLSAFB） And Bacillus polymyxa without animals

Special combination of neutral protease , each   At least it contains  250  individual  CLS  Units and  2,000  Casein unit mg dry weight . Designed for stem cell and other primary cell separation and bioprocessing applications , In these applications, it is necessary to prevent the introduction of potential pathogens of animal origin . Stored in the  2-8°C.

AI Meijie Worthington Collagenase detection ：

Gisslow  and  McBride (1975)、Robertson wait forsomeone A determination method using labeled collagen has been reported .(1972)  And Sakamoto and others .（1972）. Because the real collagenase attacks the helical region of the molecule , The change of optical rotation dispersion reflects the degradation of collagen （Keil wait forsomeone 1975）.

Worthington  Collagenase products  CLS  and  CLSPA  The determination of is as follows ：

Method ： Yes  Mandl The program of et al. Was modified .（1953  year ）. Collagenase is incubated with natural collagen  5  Hours . Use  Moore  and  Stein (1948)  Colorimetric ninhydrin method was used to determine the degree of collagen decomposition . The released amino acids are expressed in micro moles of leucine per mg of collagenase . stay  37°C  and  pH 7.5  Under specific conditions ,1  A unit is equal to  5  From collagen within hours  1  Micromore  L- Leucine equivalent .

Worthington Core enzyme ： Include ： Collagenase , Deoxyribonuclease I, Elastase , Papain , Ribonuclease , Trypsin, etc .