Human cell prosci 4-1BB ligand recombinant protein scheme

AI Meijie  ProSci 4-1BB  Ligand recombinant protein background ：

Members of tumor necrosis factor ligand superfamily  9 (4-1BBL)  Also known as  4-1BB  ligand 、CD137L  or  TNFSF9, It is a kind of  TNFRSF9  Bound cytokines .4-1BBL  yes  4-1BB  High affinity ligands .4-1BBL  Induce activated peripheral blood  T  Cell proliferation . Besides ,4-1BBL  It may be in activation induced cell death  (AICD)  It works . Besides ,4-1BBL  May be in  T  Cells and  B  cells / Macrophages play a role in homologous interactions . As for Disease ,4-1BBL It's about cancer 、 Infectious diseases and autoimmune diseases .

1： gain  EY  wait forsomeone ,2010  year ,J. Biol. chemical .285:9202-9210.

2：Alderson MR  wait forsomeone ,1994  year ,Eur.J. Immunity .24:2219-2227.

 

chart ： Immobilization  Mouse 4-1BB, His Tag at 0.5 ug/mL (100 ul/well),  It can be done with  Mouse 4-1BB Ligand, Fc Tag  combination , The linearity is  0.1-3 ng/mL.

ProSci  4-1BB  Ligand recombinant protein   chemical property ：

species ： rat

Source species ：HEK293  cells

Recombinant protein sequence ： Arginine  104 -  Glutamate  309

Fusion label ：Fc label

The test application ：E、WB

Application instructions ： The recombinant protein can be used for E、WB. For research purposes only .

Estimated molecular weight ：49 kDa

biological activity ： Through its functions  ELISA  To measure the binding capacity of . Immobilization  Mouse 4-1BB, His Tag at 0.5 ug/mL (100 ul/well),  It can be done with  Mouse 4-1BB Ligand, Fc Tag  combination , The linearity is  0.1-3 ng/mL.

AI Meijie ProSci 4-1BB  Ligand recombinant protein   characteristic ：

The purity ：SDS-PAGE  Determination  >95%.

LAL  The endotoxin level of the method is lower than  1.0 EU/ug.

Physical state ： Freeze drying

buffer ：Tris  Contains glycine 、 Arginine and  NaCl,pH7.5