Preparation of bovine serum albumin modified by low molecular weight protamine lmwp/peg-1900 on the surface of albumin nanoparticles

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Increase the hydrophobicity of albumin molecules through certain methods , Such as the reduction of disulfide bonds in protein molecules 、 Add denaturant 、 Add lipophilicity or reduce the primary amine group on the protein surface , Promote albumin self-assembly to form nanoparticles . Common reductants include β- Mercaptoethanol 、 Dithiothreitol and cysteine . The preparation process is simple , Similar to desolvent method , It is widely used .

β- There are side effects in the process of developing albumin with mercaptoethanol as reducing agent .Saleh Wait for glutathione to replace β- Hydroxyethanol to break the disulfide bond in albumin molecule , Unfold the albumin to expose the hydrophobic area , Then add an ethanol solution containing curcumin , Then form nanoparticles through self-assembly . Experiments have confirmed that albumin nanoparticles have better compatibility 、 Lower side effects .

PEG-1900 Preparation of modified bovine serum albumin ：
By activating with cyanuric chloride -PEG-1900 and PEG-5000 Prepare activated PRG. Reuse activation PEG To modify the free amino group of bovine serum albumin . Experiments show that , use PEG-1900 The free amino number of modified bovine serum albumin is 90% Left and right , It can make bovine serum albumin completely lose immunogenicity . While using PEG-5000 When decorating , The modification rate reaches 50% It can make bovine serum albumin lose the ability to produce immune antibodies in animals .

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RXYWX.2022.7.25