Prosci 14-3-3 (phosphate ser58) antibody instructions

AI Meijie ProSci 14-3-3（ phosphoric acid  Ser58） antibody background ：

14-3-3  Protein is a highly conserved protein family , It seems to play a variety of roles in cell signal transduction （Bridges  and  Moorhead,2005）. These proteins are abundantly expressed in the brain , And it has been detected in cerebrospinal fluid of patients with different nervous system diseases （Berg  etc. ,2003）.14-3-3  Protein binding protein ligand , These ligands are usually phosphorylated serine or threonine residues , And regulate the functions of these conjugated partners through a variety of different mechanisms （Silhan  wait forsomeone ,2004  year ;Dougherty  and  Morrison,2004  year ）.14-3-3  Proteins affect a variety of cellular processes , Including cell cycle and transcription 、 Signal transduction and intracellular transport .14-3-3  If these functions of proteins do not depend on the dimer structure , Can promote its role .

1：Berg D、Holzmann C、Riess O (2003) 14-3-3  Proteins in the nervous system .Nat Rev Neurosci 4：752-762.

2：Bridges D, Moorhead GB (2005) 14-3-3  protein ： Number many functions of proteins . science  STKE 2005：re10.

3：Dougherty MK, Morrison DK (2004)  Unlock  14-3-3  Password . Journal of cell science  117：1875-1884.

ProSci 14-3-3（ phosphoric acid  Ser58） antibody chemical property ：

Host species ： The rabbit

Species reactivity ： cattle ,  chicken ,  Dog ,  frog ,  human beings ,  mouse ,  Rat ,  sheep ,  Zebrafish

Immunogen ： Corresponding to rats  14-3-3  Protein phosphate -Ser58  Phosphopeptides surrounding amino acid residues .

The test application ： Immunohistochemistry 、 White blood cells

Application instructions ： The reactivity of the antibody in Western blot with human and rat tissues has been directly tested . It is expected that the antibody will interact with cattle 、 dog 、 chicken 、 Mice 、 Non human primates 、 sheep 、 Xenopus laevis reacts with zebrafish , Because these species have  100%  Homology .

Estimated molecular weight ：29

chart ： Western blotting of rat brain lysates is shown in  Ser58  Phosphorylated  ~29k 14-3-3  protein （ contrast ） Specific immune markers . Immune markers are used as phosphopeptides for antigens （Phos-pep） block , But not by the corresponding dephosphorylated peptide （ Not shown ） block .

AI Meijie ProSci  14-3-3（ phosphoric acid  Ser58） antibody characteristic ：

purification ： Affinity purification

Clonality ： Polyclonal

Physical state ： Liquid