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抗兔Dylight 488丨Abbkine通用型免疫荧光(IF)工具箱
2022-06-28 18:01:00 【Sylvia_sc】
免疫荧光技术(IF)是根据抗原抗体反应的原理,先将已知的抗原或抗体标记上荧光素,制成荧光抗体,再用这种荧光抗体(或抗原)作为探针检测组织或细胞内的相应抗原(或抗体)。

Abbkine 通用型免疫荧光(IF)工具箱实验建议:
基于传统的IF实验操作繁琐耗时、试剂用品种类繁多、实验结果稳定性可靠性差的痛点,Abbkine开发出通用型免疫荧光(IF)工具箱。可应用于组织、细胞样本的免疫荧光检测。
Abbkine通用型免疫荧光(IF)工具箱组分:
• Immunostaining Permeabilization Buffer-100 mL
• EDTA Antigen Retrieval Solution pH 8.0 (20×)-100 mL
• PBS (20×)-100 mL
• Goat Serum Blocking Buffer-20 mL
• Antibody Wash Buffer (20×)-25 mL
• Antibody Dilution Buffer-50 mL
• DyLight 488, Goat Anti-Rabbit IgG-135 μL
• DAPI (500×)-100 μL
• SuperKine Enhanced Antifade Mounting Medium-10 mL
Abbkine通用型免疫荧光(IF)工具箱特点:
• ①操作便捷,组分齐全,无需操心复杂试剂,开盒即可开始实验;
• ②提供优化的抗体稀释液和SuperKine 增强型抗荧光淬灭剂,抗淬灭效果强,维持J佳的荧光强度;
• ③提供Dylight系列二抗,具有更强的荧光强度和更高的光学耐受性及特异性;
• ④含有Dylight 488标记的山羊抗兔抗体,可以用于检测兔来源的相应一抗,观察到的颜色为鲜艳绿色。
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