Lumiprobe non fluorescent alkyne EU (5-ethynyluridine)

AI Meijie  Lumiprobe 5- Ethynyluridine  (EU)  Is used for  RNA  Common molecular biological reagents for synthetic research .

EU  It is easily absorbed by living cells and phosphorylated by pyrimidine salvage . Generated  5-ethynyl uridine-5'-triphosphate  By  RNA  Polymerase  I、II  and  III  Blend in from scratch RNA  Not uridine , But do not mix  DNA.

EU  Labeled newborn cells  RNA  It can be visualized by  Click Chemistry  Quickly and sensitively detect . Connect in decoration  RNA  Medium uridine  5  Alkynyl of position passes  Cu(I)  Catalytic azide - Cycloaddition of alkynes  (CuAAC)  React with dyes or biotin azides .

Of the tag  RNA  Different methods can be used to detect , Such as fluorescence microscopy or flow cytometry , This allows to estimate the level of transcription in cells .

5- Ethynyl uridine is superior to its analogues  5- Bromouridine , Because it is used for detection  5- Compared with the antibody of bromouridine , Dyes containing azides are very small in size and exhibit better membrane permeability . therefore ,Сlick Сhemistry  The method allows whole staining of large samples such as organ or tissue fragments .

AI Meijie Lumiprobe EU（5- Ethynyluridine ） chemical property ：

form ：  Grayish white solid

Molecular weight ： 268.22

CAS Number ： 69075-42-9

Molecular formula ： C 11 H 12 N 2 O 6

IUPAC  name ：1-((2R,3R,4S,5R)-3,4- Dihydroxy -5-( methylol ) Tetrahydrofuran -2- The base )-5- Ethynyl pyrimidine -2,4(1H,3H)- Diketone

solubility ：  dissolve in water 、DMSO、DMF

The quality control ：NMR 1 H, HPLC-MS (95%)

Storage conditions ： Store ： After receipt of  24  Months , stay  -20°C  Keep away from light . transport ： At room temperature for  3  Zhou .

AI Meijie  Lumiprobe EU（5- Ethynyluridine ） Related research ：

Alkyne hydrazide

Bifunctional reagents containing alkynyl and hydrazide functional groups . It reacts with aldehydes and ketones . Then the alkynyl group can pass through CuAAC The reaction is coupled with various azides .

Alkyne maleimide

Used to attach alkyne functional groups to proteins 、 Peptides and others with  SH（ mercaptan ） Groups （ Including cysteine residues ） On the molecule of maleimide reagent .

Alkyne  NHS  ester （ Hexylic acid  NHS  ester ）

Alkynes used in copper catalyzed click chemistry to label biomolecules  NHS  ester .

Biotin alkyne

Biotin alkyne is a reactive affinity marker , It can be connected to biological molecules by click reaction .

DBCO NHS  ester

For strain promoting copper free click chemistry  (SPAAC)  Azodibenzocyclooctyne  (DBCO, ADIBO)  Reagents . The reagent contains  NHS  Ester function , Used to attach cyclooctynes to various molecules .

DBCO STP  ester

Used to modify proteins / antibody / Active ester of peptide , Then the copper free click chemical reaction is introduced .

EdU（5- Ethynyl -2'- Deoxyuridine ）

EdU  It's a nucleoside , Reproducible by incorporation of cellular enzymes  DNA  in . after , Contained in cells  DNA  It can be obtained by mixing with the fluorescent dye azide  Click  Chemical reaction for color development , To reveal cell proliferation .

Caproic acid STP ester

Alkyne activated esters for modifying amino groups .STP  Ester group is  NHS  An excellent substitute for ester functional groups .

Pentenoic acid STP ester

A method for introducing alkynes into peptides / Protein and activated ester of click reaction .