Biovendor free light chain（ κ and λ) Test steps of ELISA Kit

Human immunoglobulin (Ig) A molecule consists of two identical heavy chains and two identical light chains , Heavy chain yes α、δ、ε、γ and μ this 5 Types , These heavy chains determine immunoglobulins, respectively IgA,IgD,IgE,IgG and IgM this 5 class ; Light chains are divided into  k (kappa) chain and λ (lambda) chain 2 Types , Every Ig There is only one type of light chain on the molecule .

FLC yes B Natural products of lymphocytes , So it represents tumor and reactivity B Unique biomarkers of cell-related diseases . FLC The increase is related to malignant plasma disorders and other lymphoid immune proliferative diseases .

meanwhile ,FLC It is also an important diagnostic aid for various monoclonal globulinopathy , For example, multiple myeloma , Macroglobulinemia , Nonsecretory myeloma , Atomized multiple myeloma , Monoclonal pneumonia , Monoclonal cranial encephalopathy . Accurate measurement of monoclonal free light chains in serum and urine is particularly important in light chain diseases , For example, light chain myeloma , Primary systemic amyloidosis and light chain deposition disease .

Quantitative monoclonal FLC The ability to monitor the disease may help . In patients with light chain myeloma , In the early stages of the disease FLC κ/λ The obvious change of the ratio . Urine monoclonal antibodies to immunoglobulins kappa or Lambda Detection of free light chains , Also known as Bence Jones protein （BJP, Protein this week ）, For identification and monitoring BCELL Malignant tumors are also important .

Let's take a look at the emcee to be introduced next  BioVendor  Human immunoglobulin free light chain (κ and λ)Elisa  Kit （ Applicable sample type ： serum 、 The plasma -EDTA、 The plasma - heparin 、 The plasma - Citrate 、 urine ）：

Free light chain (κ and λ)Elisa Kit Test steps ：

FLC kappa and FLC λ The detection range and sensitivity are respectively ：

Detection range   sensitivity

10–320ng/ml 6ng/ml

17.5–560ng/ml 5ng/m

FLC kappa and FLC λ The relative accuracy of are ：

Differences between groups ：

FLC kappa（n=6） FLC λ（n=6）

CV=7.0% CV=6.4%

Intra group differences ：

FLC kappa（n=8） FLC λ（n=8）

CV = 3.0% CV = 4.5%

AI Meijie  BioVendor  Human immunoglobulin free light chain (κ and λ)Elisa  Kit Some citation results show ：

1、Hantavirus infection-induced B cell activation elevates free light chains levels in circulation.

2、Randomized controlled trial of medium cut-off versus high-flux dialyzers on quality of life outcomes in maintenance hemodialysis patients.

3、Assessment of Intrathecal Free Light Chain Synthesis: Comparison of Different Quantitative Methods with the Detection of Oligoclonal Free Light Chains by Isoelectric Focusing and Affinity-Mediated Immunoblotting