Mee | Zhejiang University Chenglei group develops a new method for designing and constructing synthetic flora

Microorganisms are the oldest on earth 、 Most widely distributed 、 One of the most diverse forms of life . As an important component of ecosystem , Microorganisms play an important role in the material circulation and energy flow of terrestrial and marine ecosystems , Their activities are the basis of all biogeochemical cycles . In nature , Microbial individuals rarely exist alone , Instead, they gather together to form a large and highly dynamic community ; They pass through continuous substances between cells 、 Energy and information exchange , Interaction forms a complex ecological network , These complex networks are important for the composition of ecosystems 、 Structure and function are crucial . However , Limited by the complex background of natural microbial communities and the lack of effective tools to disassemble interspecific interactions , Understanding complex microbial ecological networks has always been a huge challenge .

Based on the cross integration of synthetic biology and ecology , Synthetic microbial communities are artificially constructed in a bottom-up manner , It provides a possible new perspective for exploring microbial ecological interaction networks . In recent years, there has been a little work using synthetic communities to explore interspecific interactions of microorganisms , But it is mostly limited to one or two specific interaction types , Such as cooperation and competition , It is still not enough to elucidate the complex interaction networks in natural microbial communities . therefore , A set can be compatible with different types of ecological interaction 、 And the experimental system that can effectively and systematically study the interaction between complex microorganisms remains to be developed .

Professor Cheng Lei's laboratory carried out research on how to design and construct a controllable synthetic microbial community . First , Through the combination of microbiology experimental technology and ecological theory , A system with different ecological interactions has been constructed artificially 、 Adjustable microbial experimental system ： One side , Through the transformation of engineering strains , Based on the built module unit , Assembly has synergy 、 A synthetic microbial community that interacts with competition and aggression ; On the other hand , Three different microbial dynamics model frameworks are established by using differential equations , Quantitatively describe the population dynamics of synthetic communities . secondly , Based on the successfully constructed microbial experimental system , Verify the adjustability of synthetic community and its impact on community dynamics . The results show that , Through manual regulation of two engineering modules , It can achieve synergy between the same pair of strains 、 The transformation between competition and infringement ; Besides , Synthetic communities with invasive relationships , Under certain initial conditions, the community will collapse 、 Different dynamic patterns, such as the dominance of different strains . All in all , The research results are controllable for design and construction 、 The operable microbial experimental system provides a new idea , It also opens up a possible experimental way for further ecological theory verification and mechanism discussion .

 

chart ： Amino acid metabolism and quorum sensing module unit construction

The research results are 2022 year 5 month 6 Japan In internationally renowned ecological journals 《Methods in Ecology and Evolution》 With “Synthetic microbial consortia with programmable ecological interactions” A long research article entitled （Research Article） Publish online . The College of life sciences of Zhejiang University is the first author and corresponding author unit , Professor Cheng Lei is the corresponding author of this paper , Professor Cheng Lei's laboratory doctoral student lishuyao is the first author of this article , Dr. Xiao Jing made a joint work , Dr. Xu Chenchao 、 Graduate student sun Tianzheng 、 Zhang Kaihang 、 On Fang Jian and Feng Yuantao participated in this work as co authors ; The research was supported by Professor wangbaojun, School of chemical engineering and Bioengineering, Zhejiang University . This research was supported by the National Natural Science Foundation of China for Distinguished Young Scholars 、 Major research programs and cultivation projects 、 Special funds for basic scientific research business expenses of Central Universities and Zhejiang University 2030 Ecological civilization convergence program and other funding ; Dr. Xu Chenchao is supported by the Youth Science Fund of NSFC . 

Link to the original text  ↓

https://doi.org/10.1111/2041-210X.13894

source ： Zhejiang University

Guess you like

iMeta brief introduction   Highly quoted articles   High color value drawing imageGP  Network analysis iNAP
iMeta Web tools   Metabolism group MetOrigin  Meggie cloud Lactation prediction DeepKla
iMeta review   Intestinal flora   Plant flora   Oral flora   Protein structure prediction

10000+： Flora analysis   Babies and dogs   Rhapsody of syphilis   carry DNA Hair Nature

Series of tutorials ： Introduction to microbiome  Biostar  Microbiome    Macro genome

Expertise ： Academic charts   High score article   Shengxin Scripture   An indispensable person

Article to read ： Macro genome   Parasite benefits   Evolutionary tree   Necessary skills ： put questions to   Search for   Endnote

Amplification analysis ： Chart interpretation   Analysis process   Statistical mapping

Biological science :   Intestinal bacteria   Life on the human body   Great leap forward in life    Cell warfare   The mystery of human body   

Written in the back

To encourage readers to communicate and quickly solve scientific research difficulties , We established “ Macro genome ” Discussion groups , There are already domestic and foreign 6000+ Researchers join . Please add editor in chief wechat meta-genomics Bring you into the group , Be sure to remark “ full name - Company - Research direction - The title / grade ”. Please indicate your identity for the senior title , There are also microorganisms at home and abroad PI Cooperation and exchange of group supply . Ask for help with technical problems , First read 《 How to ask questions gracefully 》 Learn how to solve problems , Unresolved intra group discussions , Don't talk about problems in private , Help colleagues .

Click to read the original text , Jump to the latest article catalog to read