Lumiprobe click chemistry - non fluorescent alkyne: hexanoic acid STP ester

AI Meijie Lumiprobe  Non fluorescent alkynes ： Caproic acid STP ester background ：

Alkyne activated ester , Used to label proteins 、 Amino groups in peptides and other molecules with aliphatic amine functional groups .

Sulfo tetrafluorophenyl  (STP)  Esters are traditional  N- Hydroxysuccinimide  (NHS)  An excellent substitute for esters . And  NHS  Ester comparison ,STP  Esters provide better labeling and hydrolysis ratios in the aqueous environment .

Hexenoic acid fragment is a short connector with amine . For longer amine connections , Consider using alkynes -PEG  Activated ester .

AI Meijie Lumiprobe  Non fluorescent alkynes ： Caproic acid STP ester chemical property ：

form ：  White solid

Mass spectrometry  M+  The incremental ： 94.04

Molecular weight ： 362.23

Molecular formula ： C 12 H 7 F 4 O 5 SNa

IUPAC  name ： 4-(hex-5-ynoyloxy)-2,3,5,6- Sodium tetrafluorobenzene sulfonate

solubility ：  dissolve in water 、DMF、DMSO

The quality control ： NMR 1 H, HPLC-MS (95%)

Storage conditions ：  Store ： After receipt of  12  Months , stay  -20°C  Keep away from light . transport ： At room temperature for  3  Zhou . Avoid prolonged exposure to light . dry .

AI Meijie Lumiprobe  Non fluorescent alkynes ： Caproic acid STP ester Related research ：

Pentenoic acid STP ester

Used to introduce alkynes into peptides / Activated esters of proteins .

EU（5- Ethynyluridine ）

Uridine alkyne derivatives are used to monitor and study  RNA  Transcription .EU  Labeled cells  RNA  With fluorescent dyes or biotin azides  CuAAC  Reaction and subsequent fluorescence measurements .

EdU（5- Ethynyl -2'- Deoxyuridine ）

EdU  It's a nucleoside , Reproducible by incorporation of cellular enzymes  DNA  in . after , Contained in cells  DNA  It can be obtained by mixing with the fluorescent dye azide  Click  Chemical reaction for color development , To reveal cell proliferation .

DBCO STP  ester

Used to modify proteins / antibody / Active ester of peptide , Then the copper free click chemical reaction is introduced .

DBCO NHS  ester

For strain promoting copper free click chemistry  (SPAAC)  Azodibenzocyclooctyne  (DBCO, ADIBO)  Reagents . The reagent contains  NHS  Ester function , Used to attach cyclooctynes to various molecules .