Laboratory procedures and references of chloramphenicol acetate

AI Meijie   Cayman biological chloramphenicol acetate Chemical properties of ：

CAS Registry No.: 10318-16-8

Formal Name:

 N-[(1R,2R)-1-[(acetyloxy)methyl]-2-hydroxy-2-(4- nitrophenyl)ethyl]-2,2-dichloro-acetamide

MF: C  H  Cl N O

13   14   2   2   6

FW: 365.2

Purity: ≥97%

Supplied as: A solid

Storage: -20°C

Stability: ≥2 years

Item Origin: Streptomyces sp.

AI Meijie   Cayman biological chloramphenicol acetate laboratory procedure ：

Chloramphenicol acetate is supplied in solid form . The stock solution can be prepared by dissolving chloramphenicol acetate in the selected solvent . Chloramphenicol acetate is soluble in ethanol 、 methanol 、 Dimethyl sulfoxide, dimethylformamide and other organic solvents , Purge with inert gas . In an acidic solution , Acetate may migrate to benzyl alcohol .

Chloramphenicol acetate is slightly soluble in aqueous solution . In order to improve water solubility , Dilute the organic solvent solution into water buffer or isotonic brine . If biological experiments are carried out , Please ensure that the residual amount of organic solvents is negligible , Because organic solvents may have physiological effects at low concentrations . It is not recommended to store the aqueous solution for more than one day .

About the description ：

Chloramphenicol acetate is an acetylated and inactive version of chloramphenicol .2 In Escherichia coli and Staphylococcus aureus , Acetyl coenzyme A（ Acetyl coenzyme A; Project number 16160） In the presence of , Formed by the inducible enzyme chloramphenicol acetyltransferase , To produce chloramphenicol resistance . In turbidity measurement , Chloramphenicol acetate has no antibacterial activity against Streptococcus sonnei .

AI Meijie   Cayman biology   Chloramphenicol acetate references ：

1.Fodor, G., Toth, J., Kovacs, E., et al. Synthesis of chloramphenicol. Russian Chemical Bulletin 4, 391-399 (1995).

2.Shaw, W.V. and Brodsky, R.F. Characterization of chloramphenicol acetyltransferase from chloramphenicol-resistant Staphylococcus aureus. J. Bacteriol. 95(1), 28-36 (1968).