Study on lumiprobe modified triphosphate biotin-11-utp

Triphosphate in the reaction group

Nucleic acid such as  DNA  and  RNA  Enzymatic modification can be carried out by using the modified nucleoside triphosphate . Primer extension 、 Notch translation and  PCR  Is an example of an enzymatic reaction that can be used for labeling . AI Meijie  Lumiprobe  Contains a series of functionalized triphosphates , In order to realize the effective labeling of nucleic acids .

AI Meijie  Lumiprobe Biotin-11-UTP  yes  RNA  Polymerase  SP6、T3  and  T7  The substrate of . Biotinylated triphosphate is natural  UTP  Analogues , It can be used in in vitro transcription  RNA  Mark .

In many applications , Biotin labeled  RNA  It can replace radioactive marks  RNA, Include  Northern  and  Southern  Imprint 、 In situ hybridization and microarray analysis . Detection of biotinylated by various methods using streptavidin conjugates  RNA,11  Long junctions of atoms allow for efficient interaction between biotin and streptavidin .

 

AI Meijie  Lumiprobe Biotin-11-UTP  chemical property ：

form ：  Colorless solid

Molecular weight ： 894.48

Molecular formula ： C 28 H 43 N 6 Li 3 O 18 P 3 S

IUPAC  name ： ((2R,3S,4R,5R)-5-(2,4-dioxo-5-(3-(6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3, 4-d]imidazol-4-yl)pentanamido)hexanamido)prop-1-yn-1-yl)-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl Hydrogen triphosphate

solubility ：  Good water

The quality control ： HPLC-MS (95%), Enzymatic reaction test

Storage conditions ：  Store ： stay  -20°C  Keep away from light . transport ： At room temperature for  3  Zhou . Avoid excessive freeze-thaw cycles .

 

AI Meijie  Lumiprobe Biotin-11-UTP  Related research ：

amino -11-ddUTP

Amino-11-ddUTP  It is used to decorate  DNA  Triphosphate .

amino -11-dUTP

Amino-11-dUTP  It is used to decorate  DNA  Triphosphate .

amino -11-UTP

Uridine triphosphate labeled with amino group , It is used for indirect non radioactive enzyme catalysis  RNA  Mark .

Biotin -11-dCTP

For non radioactive  DNA  Labeled biotinylation  dCTP.

Biotin -11-dUTP

Biotin labeled deoxyuridine triphosphate , For enzymatic incorporation  DNA  And indirect detection .

FAM-11-dCTP,6- Isomer

For enzymatic  DNA  Of the tag  6-FAM  Of the tag  dCTP.

FAM-11-dUTP,6  Isomer

be used for  DNA  Marked with green emission  dUTP  Of  FAM  derivatives .

FAM-11-UTP,6  Isomer

6-FAM- Uridine triphosphate is used as a non radioactive enzyme in the process of transcription in vitro  RNA  Mark .

Sulfo cyanine 3 dUTP

Used for marking with sulfo cyanine  DNA  The fluorescence of  dNTP. Nucleotides contain a long linker , Allow to include  Taq  Various enzymes, including polymerases, effectively incorporate dyes into  DNA  In the chain .

Sulfo cyanine 5 dUTP

Sulfo-Cyanine5 dUTP  It is a fluorescent labeled triphosphate , To use  sulfo-Cyanine5  Fluorophore pair  DNA  Carry out enzymatic modification . The reagent contains a long connecting arm , Its incorporation efficiency is very high .

Sulfo-Cyanine5.5 dUTP

Containing sulfo -Cyanine5.5（ also called  Cy5.5） Modified deoxytriphosphate of dyes , be used for  DNA  Microarrays and  PCR  Enzyme labeling of amplicons and reverse transcripts .