Easy gene ｜ introduction to macrovirus sequencing technology

Hello everyone , This is more than ten years of focusing on epigenomics , Easy genes leading the research service of multi omics .

Viral metagenomics （Meta-virome） It is based on the theory of metagenomics , Combined with the existing detection technology of virus molecular biology, it is a new branch of Science , It is all viruses in a certain kind of sample (virus) Or viral analogues (virus-like-particle) And the genetic information it carries . The macrovirus group directly studies the genetic material of all viruses in the environment , It can quickly and accurately identify all virus components in the environment , Found in the virus 、 Trace the source of the virus 、 Research on microbial early warning plays an important role . Macrovirus research can be applied to human or animal intestinal tract or blood samples 、 ocean 、 Research on soil, etc , To explore potential hazards to human beings and the environment .

Easy gene technology and the well-known microbiology research group of the University of Copenhagen in Denmark jointly developed an efficient macrovirus particle enrichment method , Virus particles can be enriched in a small amount of samples , And ensure virus activity and high homogeneity , For macroviromics 、 The research on the interaction between bacteriophages and bacteria provides excellent technical support , Escort ！

Technical advantages ：

Enrichment of virus particles in samples , Realize the effective separation of virus groups in micro samples .
No damage to phage , The virus activity is guaranteed to the greatest extent , Suitable for downstream FVT Research .
Compared with traditional PEG,CsCl Other methods , High homogeneity , Phage preference is low .
Comprehensive data analysis strategy , Customized multiomics association data mining .

Research direction ：

Macroviromics research in Virus discovery 、 Trace the source of the virus 、 Research on microbial early warning plays an important role ：

Agriculture ： Environmental pollution 、 Ecosystem damage and crop residual chemicals
Ecological environment ： Regulating host community structure 、 Participate in elemental geochemical cycle 、 Gene level mobile media
Medicine ： Research on phage and phage therapy 、 Human enterovirus group research

technology roadmap ：

Experiment strategy ：

Analysis content ：

 

Sample delivery requirements ：

Classic case

Medical direction （ Cooperative team research ）

Bacteriophages are the main members of the faecal virus group , Fecal virus group transplantation （FVT） Or it can effectively regulate intestinal flora .Gut The latest release came from a team from the University of Copenhagen in Denmark （ Easy gene partners ） The study of , In this proof of concept experiment, it is found that , After receiving the fecal virus group of lean mice , The weight gain of mice fed high-fat food was slower , At the same time, it can maintain normal blood glucose index ,FVT These protective functional metabolic effects are mediated by changes in intestinal flora . This study shows that ,FVT Therapy may be used to improve metabolic diseases related to intestinal flora, such as obesity and diabetes .

Agronomy ：

Viral and microbiome analysis of pig fecal samples showed , Bacteriophages and protophages rarely carry antibiotic resistance genes

Billaud,et al.(2021). Analysis of viromes and microbiomes from pig fecal samples reveals that phages and prophages rarely carry antibiotic resistance genes. ISME Communications. 1. 55. 10.1038/s43705-021-00054-8.

1. background ：

Understand antibiotic resistance genes （ARGs） The spread of is crucial to human health . So , It is necessary to determine which type of mobile genetic element （MGEs） Can transmit them from animal hosts to human pathogens . Previous studies have shown that , In pig feces , Antibiotic resistance genes （ARGs） It may be encoded by bacteriophages . However, due to bacteria DNA pollution problem , There is still a lack of convincing evidence to prove that the phage coding in the swine virus group ARGs.

2. Method ：

collect 14 Fecal samples of pigs or piglets , Use standard procedures to purify the virus group （VP） And total microbiome （P） Conduct DNA Extract for deep sequencing analysis , To study phage and protophage encoded ARGs.

3. Conclusion ：

The results of this study show that ARGs It does not exist in the phage genome formed by active viral bodies （ Lower than the virus overlap in the virus group 0.02%）, But it exists in three protophages , Of the viral overlaps identified in the microbiome dataset 0.02%. However, no corresponding phage was detected in the virus group , Its genetic map shows that there may be defects . The results showed that in pig fecal samples , Bacteriophages and protophages are rarely carried ARGs. Besides , This data is the first comprehensive understanding of the interaction between pre phage and virus particles , And reveals inoviruses and Oengus-like Two major bacteriophage branches .

It was found in the weight overlap of phages and protophages in pig feces samples ARGs

reference ：

① Faecal virome transplantation decreases symptoms of type 2 diabetes and obesity in a murine model DOI: 10.1136/gutjnl-2019-320005

② Billaud,et al.(2021). Analysis of viromes and microbiomes from pig fecal samples reveals that phages and prophages rarely carry antibiotic resistance genes. ISME Communications. 1. 55. 10.1038/s43705-021-00054-8.

Related reading ：

3 Text focus ： Research on the application of macrovirome sequencing in enteropathy

blockbuster ！ Viral metagenomics （Meta-virome） Come on. ~

How to do multiomics Association Research ？DNA Methylation group + Transcriptome + Macro genome +16S Research ideas

What does metagenome sequencing bring us ？

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