Application parameters of Southern biotech HRP labeled avidin avidin avidin

HRP Mark Avidin Avidin neutrophil avidin ：

Cat. No. Format Size

7200-01 Purified (UNLB) 5.0 mg

7200-02 Fluorescein (FITC) 1.0 mg

7200-03 Rhodamine (TRITC) 1.0 mg

7200-04 Alkaline Phosphatase (AP) 1.0 mL

7200-05 Horseradish Peroxidase (HRP) 1.0 mL

7200-06 β-galactosidase (BGAL) 1.0 mL

7200-07 Texas Red (TXRD) 1.0 mg

7200-09 R-phycoerythrin (PE) 0.5 mg

7200-11 Allophycocyanin (APC) 0.1 mg

7200-12 Cyanine 3 (CY3) 0.1 mg

7200-13 Spectral Red (SPRD) 0.1 mg

7200-15 Cyanine 5 (CY5) 1.0 mg

AI Meijie  SouthernBiotech  HRP Mark Avidin Avidin description ：

Specificity ： Biotin

source ： neutralization （pI=6.3±0.3）, Purify deglycosylated avidin from egg white

 

AI Meijie  SouthernBiotech  HRP Mark Avidin Avidin   application ：

Applications that have been tested for quality include ——

ELISA

FLISA

FC

Other reference applications include ——

ELISPOT

IHC-FS

IHC-PS

ICC

WB

Microarray

 

AI Meijie  SouthernBiotech  HRP Mark Avidin Avidin work As diluent ：

Flow cytometry ：

FITC and CY3 Conjugate ≤ 1µg/106 Cells

PE、APC、SPRD and CY5 Conjugate ≤ 0.2µg/106 Cells

For flow cytometry , It is recommended that the final volume of these reagents be 100µL

FLISA：

FITC、TRITC、TXRD and CY3 Conjugate 1:100–1:400

PE、APC and CY5 Conjugate ≤ 1 MCG / ml

ELISA：

AP conjugate 1:500–1:2000

HRP conjugate 1:4000–1:8000

BGAL conjugate 1:500

SouthernBiotech HRP Mark Avidin Avidin partial references ：

1. Brown MP, Topham DJ, Sangster MY, Zhao J, Flynn KJ, Surman SL, et al. Thymic lymphoproliferative disease after successful correction of CD40 ligand deficiency by gene transfer in

mice. Nat Med. 1998;4:1253-60. (ELISA)

2. Reinagel ML, Taylor RP. Transfer of immune complexes from erythrocyte CR1 to mouse macrophages. J Immunol. 2000;164:1977-85. (ELISA, FC)

3. Hodge LM, Marinaro M, Jones HP, McGhee JR, Kiyono H, Simecka JW. Immunoglobulin A (IgA) responses and IgE-associated inflammation along the respiratory tract after mucosal

but not systemic immunization. Infect Immun. 2001;69:2328-38. (ELISA, ELISPOT)