Lumiprobe copper free click chemical solution

Copper free click chemical reactions include cycloaddition reactions for biological conjugation , It is different from traditional copper catalyzed click chemistry , It does not depend on the use of transition metal catalysts .

These reactions include strain promoted alkynes - Azide cycloaddition  (SPAAC), These involve strained cycloalkynes and azides . The mitigation of spatial strain provides the driving force for this reaction .

DBCO (ADIBO)  Cycloalkynes are the most reactive but stable . The reaction produces two regiosequitomers that are usually not separable , So this does not lead to  HPLC  Double the peak in .

Another process is  TCO  Connect （ Tetrazine  - Trans cyclooctene linkage ）, It is also powered by spatial strain . And  SPAAC  Different , This is a  [4+2]- Cycloaddition generation  6  Elementary ring .TCO  Ligation is one of the fastest reactions for biological coupling .

Both reactions are very biocompatible , Because they do not require the use of metal catalysts , They're fast , And in  DBCO  The case of cycloalkynes is orthogonal （ namely , Two reactions can be carried out in a mixture without interference ）.

AI Meijie  Lumiprobe  A variety of copper free click chemicals , Apply to  SPAAC  and  TCO  Connect .

AI Meijie  Lumiprobe BDP FL DBCO：

BDP FL DBCO  It is a kind of dye cyclooctyne , For copper free click chemical reaction with azide .BDP FL  The absorption and emission wavelengths correspond to fluorescein  (FAM)  Absorption and emission wavelengths , but  BDP FL  The photostability of is obviously higher .

BDP FL  It's a  FAM（ fluorescein ） Photostable boron Dipyrrole methylene of the channel . The dye is compatible with the fluorescein filter set .

This is used for copper free click chemical reaction with azide  DBCO（ Azadibenzocyclooctyne ） derivatives .

AI Meijie  Lumiprobe BDP FL DBCO chemical property ：

form ：  Orange solid

Mass spectrometry  M+  The incremental ： 592.3

Molecular weight ： 592.49

CAS Number ： 2360493-46-3

Molecular formula ： C 35 H 35 N 4 BF 2 O 2

solubility ：  Good at  DMF、DMSO、DCM

The quality control ：NMR 1 H, HPLC-MS (95%)

Spectral characteristics

Maximum excitation / absorption ,nm： 503

ε, L⋅mol -1 ⋅cm -1： 92000

Maximum emission , nanometer ： 509

Fluorescence quantum yield ： 0.97

CF 260： 0.015

CF 280： 0.027

AI Meijie  Lumiprobe  Copper free click chemistry Related research ：

BDP 581/591  Alkyne

BDP 581/591  It is a boron Dipyrrole methylene fluorophore , It can be used for fluorescence polarization measurement 、 Microscopic examination and two photon experiments . The alkyne is a derivative of copper catalyzed click chemistry .

The flowers are green 5 Tetrazine

Cyanine5  It is a universal red luminous dye , It has very high extinction coefficient and good quantum yield . This tetrazine derivative reacts very rapidly with trans cyclooctene and cyclopropene , Form stable conjugates .

The flowers are green 5 DBCO

Dibenzocyclooctynes for copper free click chemistry  (DBCO)  Functional  Cyanine5  dyestuff .