Lumiprobe biotin phosphimide (hydroxyproline) instructions

AI Meijie Lumiprobe  Application of biotinylation ：

Biotinylation is used to modify biomolecules with biotin labels , It can then be detected with streptavidin or avidin .Lumiprobe  Provide a range of products with  PEG  Chemical or  X  Biotin reagent of spacer , And for binding primary amines 、 Sulfhydryl group 、 Carboxyl groups and various reactive groups in click chemistry .

AI Meijie Lumiprobe Biotin phosphimide （ hydroxyproline ） background ：

Non nucleoside modifiers for the synthesis of biotinylated oligonucleotides based on hydroxyproline core . The phosphorous amide contains a dimethoxytriphenyl methyl protecting group , Used in  C 18 Column and reversed phase  HPLC  Oligonucleotides obtained by purification on . The modifier reagent can be used to introduce biotin  5'- position 、3'- position （ Use generic  CPG） And internal oligonucleotide sites .

The amides do not require special condensation and deprotection conditions .

 

AI Meijie  Lumiprobe Biotin phosphimide （ hydroxyproline ） chemical property ：

form ：  White powder

Molecular weight ： 959.18

Molecular formula ： C 51 H 71 N 6 O 8 PS

solubility ：  Good at  MeCN、DCM、THF

The quality control ：NMR 1 H、31 P、HPLC-MS (95%)、 Functional tests in oligonucleotide synthesis

Storage conditions ： Store ： stay  -20°C  Keep away from light . transport ： At room temperature for  3  Zhou . dry .

Details of oligonucleotide synthesis

Dilute ：  acetonitrile

Coupling conditions ： Standard coupling , Same as normal nucleobase

Deprotection condition ： Same as the protected nucleobase

AI Meijie  Lumiprobe  Biotin phosphimide （ hydroxyproline ） Related research ：

Biotin alkyne

Biotin alkyne is a reactive affinity marker , It can be chemically linked to biomolecules by clicking .

Biotin -PEG3- Azide

Biotin azide  It is a kind of biotin （ A well-known affinity probe ） Click on the chemically labeled reagent .