Peptide nucleic acid oligomer containing azobenzene monomer (nh2-tnt4, n-pnas) Qiyue biological customization

Peptide nucleic acid oligomers containing azobenzene monomers (NH2-TNT4,N-PNAs) Qiyue biological customization

PNA Double chains can be formed in either direction , But the antiparallel orientation takes precedence , Form ordinary double chain . For antisense and DNA Probe type application , Antiparallel is the preferred conformation . When antiparallel orientation ,PNA The probe's N End equivalent DNA Of 5’ End .

·PNA The melting point is different from DNA. because PNA The chain is uncharged ,PNA-DNA Of Tm The value will be higher than the corresponding DNA-DNA The height of . Usually ,100mMNaCl in , Every additional base , Its Tm Value increased by about 1°C. At a lower salt concentration , Its Tm The difference in values will be obvious . Usually , One containing 10 Base PNA, Its Tm Value about 50°C, contain 15 One base PNATm Value about 70°C.

The length is 12～17 One base PNA Sequence . The length of the sequence mainly depends on its specific application . And DNA Compared with ,PNA Probe sequences require shorter lengths . Long chain PNA, According to the sequence , Tend to aggregate , Not easy to purify and characterize . However , The shorter the sequence , The worse the specificity . therefore , For short sequences , The impact of mismatches is even greater .

 

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Small make up axc,2022.07.20