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含有偶氮苯单体的肽核酸寡聚体(NH2-TNT4,N-PNAs)齐岳生物定制
2022-07-27 05:31:00 【qiyueshengwu】
含有偶氮苯单体的肽核酸寡聚体(NH2-TNT4,N-PNAs)齐岳生物定制
PNA可以在任一方向形成双链,但逆平行取向优先,形成普通双链。对于反义和DNA探针类型应用,逆平行是优先构象。当逆平行取向时,PNA探针的N端相当于DNA的5’端。
·PNA熔点不同于DNA。由于PNA链是不带电的,PNA-DNA的Tm值会比相应DNA-DNA的高。通常情况下,100mMNaCl中,每增加一个碱基,其Tm值提高约1°C。在较低盐浓度下,其Tm值的差异将明显。通常,一个含10个碱基PNA,其Tm值约50°C,含15个碱基的PNATm值约70°C。
长度为12~17个碱基的PNA序列。序列长度主要取决于其具体应用。与DNA的应用相比,PNA探针序列需要的长度更短。链长的PNA,根据序列,倾向于聚合,不易纯化和表征。然而,序列越短,特异性越差。因此,对于短序列来说,不匹配的影响更大。

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小编axc,2022.07.20
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