Pcr/qpcr research: lumiprobe dsgreen is used for real-time PCR

AI Meijie Lumiprobe dsGreen  Is a very sensitive  dsDNA  Detection dye .dsDNA  The high sensitivity and high selectivity of  dsGreen  As  qPCR  Common to  dsDNA  test kit . No marking probe is required to detect  dsGreen  Amplification of —— Unlabeled primers are sufficient .

And  Lumiprobe  Others for gel dyeing  dsGreen  Different preparations , This formulation is specifically designed for real-time use  PCR  experiment . The specific features are ：

1、 Dye concentration for  qPCR  optimized , And carefully adjust the repeatable results between batches

2、PCR  Test preparation – QA

3、 Low fluorescent background  -  High fluorescence intensity gain

AI Meijie  Lumiprobe dsGreen  For real time  PCR,100× chemical property ：

form ： Pale orange solution

The quality control ：NMR 1 H、HPLC-MS (95%)、PCR  test

Storage conditions ： Stored in the  -20°C  Keep away from light . transport ： At room temperature for  3  Zhou . Avoid prolonged exposure to light .

AI Meijie Lumiprobe dsGreen  For real time  PCR Spectral characteristics ：

The greatest inspiration / absorption ,nm： 454

ε, L⋅mol -1 ⋅cm -1：73000

The largest launch , nanometer ：524

Fluorescence quantum yield ：0,8

AI Meijie LumiprobedsGreen  For real time  PCR Related research ：

dsGreen  Gel staining solution ,10000×

dsGreen  For dyeing in gel  dsDNA.

dsSafe  Gel staining solution  10,000×

be used for  DNA  and  RNA  Fluorescent dyes for visualization of gel , It is the best and safer substitute for ethidium bromide .

PCR/qPCR  Premixed liquid ,2x

Pico488 dsDNA  Quantitative reagent ,200x DMSO  solution

Pico488  It's a dye , Used in the presence of  RNA、ssDNA  And other impurities  dsDNA  Perform more accurate quantification based on fluorescence . The dye shows bright fluorescence in the fluorescein channel , At low  pg/mL  Supreme  ng/mL  Within the scope of  DNA  Concentration provides a linear response .

QuDye dsDNA BR  Reagents ,200x

be used for  qPCR  Of  ROX  Reference dyes

ROX  Is used for  qPCR  Amplified passive reference dye . The dye can be used for standardization  qPCR  Report the fluorescence intensity of the dye in .