Abbkine column exkine Pro animal cell / tissue Total Protein Extraction Kit

Preparation of extracts from animal cells or tissues , The extracted protein can be used for SDS-PAGE、Immunoblottings、IP、ELISA、 Enzyme detection and other applications such as protein analysis and small-scale protein chromatography purification .

Abbkine ExKine Pro Animal cell / The tissue total protein extraction kit provides a simple 、 A convenient method for extracting total protein from cultured cells or fresh tissues .

Abbkine ExKine Animal cell / Tissue total protein extraction kit components ：

•Denaturing cell lysis buffer

• Native cell lysis buffer

• Protein extraction filter cartridges

• Collection tubes with cap

• Plastic rods

Abbkine ExKine Animal cell / Characteristics of tissue total protein extraction kit ：

• Multi purpose — Suitable for fresh mammalian cell or tissue samples , Almost no cross contamination .

• Convenient and quick — Non denaturation , Active proteins can be found in less than 7 Minutes to quickly extract .

• Compatibility is good. — The extracted protein is suitable for a variety of downstream experiments , Such as Western Blot, Protein analysis, etc .

Precautions in use ：

All the experimental steps should be in 2-8°C perform . Use pre cooled buffer and equipment , And ensure that all solutions are thawed and uniform .

chart 1 Protein map of mouse liver extracted from denatured lysis buffer and natural buffer of different manufacturers . The extracted protein was extracted with 10%SDS-PAGE Separate , Coomassie blue staining . Vehicle Lane 1, Mark ; passageway 2, Native buffer provided by the manufacturer ; Vehicle Lane 3,Abbkine Local buffer for ; Vehicle Lane 4, Denatured cracking buffer provided by the manufacturer ; passageway 5,B Denatured cracking buffer provided by the manufacturer ; Vehicle Lane 6,C Manufacturer's denatured cracking buffer ; Vehicle Lane 7, come from Abbkine Denatured cleavage buffer ; Vehicle Lane 8,D Denatured cracking buffer provided by the manufacturer .