Biovendor free light chain（ κ and λ) Chemical properties of ELISA Kit

Human immunoglobulin molecules are defined by the immunoglobulin class （IgG、IgA、IgM、IgD  and  IgE） And the same light chain covalently linked with the heavy chain （κ or λ） form . In healthy individuals , Most light chains in serum bind to heavy chains . However , Low levels of free light chains were found in the sera of normal individuals  (FLC), This is due to maturity  B  Cells produce too many heavy chains . In the serum ,FLC kappa  Mainly in the form of monomer , The molecular weight is  22.5 kDa,FLC λ Mainly in the form of dimer , The molecular weight is  45 kDa. This size difference leads to different glomerular filtration rates , So in the serum  FLC κ And  FLC λ The ratio is  1:1.6.

Also observed in urine  FLC, However, the filtration and reabsorption of low molecular weight proteins in the kidney have a strong impact  FLC  concentration , So the urine of healthy individuals  FLC  The level is low .

FLC  yes  B  Natural products of lymphocytes , So it represents tumor and reactivity  B  Unique biomarkers of cell-related diseases . To increase the  FLC  It is related to malignant plasma cachexia and other lymphocyte related immune proliferative diseases .

FLC  The detection of is an important auxiliary means for the diagnosis of a variety of monoclonal gammaglobulinopathy , For example, multiple myeloma 、Waldenstrom  Macroglobulinemia 、 Nonsecretory myeloma 、 Smoking multiple myeloma 、 Monoclonal gammaglobulinopathy of unknown significance . Accurately measure serum and / Or monoclonal free light chains in urine are particularly important in light chain diseases , For example, light chain myeloma 、 Primary systemic amyloidosis and light chain disease . Quantitative monoclonal  FLC  The ability to monitor diseases may help . In patients with light chain myeloma , It mainly produces light chains 、κ or λ, And lead to the early stage of the disease  FLC κ/λ A significant change in the ratio . Detection of immunoglobulin monoclonal antibodies in urine κ or λ Free light chain ,

Besides , Compared with health , In some inflammatory or autoimmune diseases （ Such as systemic lupus erythematosus 、 Rheumatoid arthritis or multiple sclerosis and cancer ） And  B  In the case of cell activation , Polyclonal  FLC  The synthesis of 、 Diabetes, etc ）.

AI Meijie  BioVendor  Human immunoglobulin free light chain (κ and λ)Elisa  Kit —— Applicable sample type ： serum 、 The plasma -EDTA、 The plasma - heparin 、 The plasma - Citrate 、 urine .

AI Meijie  BioVendor Free light chain (κ and λ)Elisa Chemical properties of the kit ：

type ： Sandwich  ELISA, Biotin labeled antibody

application ： serum 、 The plasma -EDTA、 The plasma - heparin 、 The plasma - Citrate 、 urine

Sample requirements ：2×5 µl/ hole

Storage / expire ： Store the kit in  2–8°C. under such conditions , The complete kit is stable before the expiry date （ See the label on the box ）.

Calibration range ：

10–320 µg/l (FLC kappa)

17.5–560 µg/l (FLC λ)

Detection limit ：

FLC kappa ELISA  Detection limit  (LOD)（ It is defined as the concentration of analyte whose absorbance is higher than the average absorbance of blank plus three standard deviations of blank absorbance ：Ablank + 3×SDblank） From the actual  FLC kappa  It is calculated that , And for  6  MCG / l .

FLC λ ELISA  Detection limit  (LOD)（ It is defined as the concentration of analyte whose absorbance is higher than the average absorbance of blank plus three standard deviations of blank absorbance ：Ablank + 3×SDblank） From the actual  FLC λ Value calculation , And for  5 μg/L.

AI Meijie  BioVendor Human immunoglobulin free light chain (κ and λ)Elisa  Kit features ：

For research purposes only .

The total determination time is less than  3.5  Hours .

The kit can measure serum 、 In plasma and urine  FLC kappa  and  FLC lambda.

Kit components are available for immediate use 、 Concentration and freeze-drying .

Calibrators and quality control products are based on human serum .

Human immunoglobulin free light chain (κ and λ)Elisa  Kit Related research ：

According to the research topic ：

Autoimmunity

oncology

By numerator ：

Immunoglobulin free light chain  Kappa  and  Lambda