中英文说明书丨Abbkine AbFluor 488-鬼笔环肽

Background：

Phalloidin belongs to a class of toxins called phallotoxins, which are isolated from the death cap mushroom (Amanita phalloides). It is a bicyclic peptide that binds to F-actin specifically. Therefore, the distribution of F-actin can be very conveniently studied by using a fluorescent dye-labeled phalloidin. Inside the phalloidin, there is an unusual thioether bridge between cysteine and tryptophan, which can form an inner ring structure. When the pH is raised, the thioether is cleaved and the phalloidin loses its affinity for actin.

Applications notes：

AbFluor 488-Phalloidin selectively bound to F-actins, it is much higher photostability than the fluorescein-phalloidin conjugates. Phalloidin derivatives are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, which can stain F-actins in cell cultures or cell-free experiments at nanomolar levels.

Features & Benefits：

•Optimized staining protocol for labeling, identifying and quantitating F-actin in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments.

• Proprietary AbFluor 488-Phalloidin (Green Fluorescence) (Ex/Em = 490/515 nm)-high-affinity probe for F-actins and much higher photostability than the other fluorescein-phalloidin conjugates.

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Abbkine AbFluor 488-鬼笔环肽背景：

鬼笔环肽（phalloidin），是从一种剧毒磨菇(Amanita phalloides)中分离出来的一种多肽物质，属于毒伞肽类毒素，是一种强烈毒素。它是一种特异性结合F-肌动蛋白的双环肽，因此，使用荧光染料偶联的鬼笔环肽可以非常方便地研究F-actins的分布。在鬼笔环肽内部，半胱氨酸和色氨酸之间存在不寻常的硫醚桥，可形成内环结构。当pH升高时，硫醚被裂解，鬼笔环肽丧失对肌动蛋白的亲和力。

Abbkine AbFluor 488-鬼笔环肽实验建议：

AbFluor 488-鬼笔环肽特异性地结合F-actins，与荧光素-鬼笔环肽偶联物相比，具有更强的光稳定性。使用nM浓度的鬼笔环肽衍生物，即可便捷地标记，鉴定，定量F-actins，适用于甲醛固定与透化处理的组织切片、细胞培养物或无细胞实验。

Abbkine AbFluor 488-鬼笔环肽特点：

• 优化的实验方案，特别适用于甲醛固定与透化处理的组织切片、细胞培养物或无细胞实验中的F-actins标记，鉴定以及定量需求。

• zhuanli的AbFluor 488-鬼笔环肽 (绿色荧光) (Ex/Em = 490/515 nm)，特异性地结合F-actins，且兼具比其它荧光素-鬼笔环肽偶联物更强的光稳定性。

使用注意事项：

鬼笔环肽为有毒物质，请小心处理。